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Mouse Anti-PRDM9 Recombinant Antibody (CBYC-P596) (CBMAB-P2723-YC)

Provided herein is a Mouse monoclonal antibody against Human PR/SET domain 9. The antibody can be used for immunoassay techniques, such as IP.
See all PRDM9 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-P596
Antibody Isotype
IgG1
Application
IP

Basic Information

Immunogen
195-385 of 894 of PRDM9 Protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS with 0.09% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
PR/SET domain 9
Introduction
PRDM9 is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity.
Entrez Gene ID
UniProt ID
Alternative Names
KMT8B; MEISETZ; MSBP3; PFM6; ZNF899
Function
Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination (PubMed:24634223, PubMed:24095733, PubMed:26833727, PubMed:27129774).
Can also methylate all four core histones with H3 being the best substrate and the most highly modified (PubMed:24095733, PubMed:24634223, PubMed:26833727).
Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate (By similarity).
During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity (PubMed:26833727).
Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression (By similarity).
During meiotic progression hotspot-bound PRDM9 interacts with several complexes; in early leptonema binds CDYL and EHMT2 followed by EWSR1 and CXXC1 by the end of leptonema. EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity).
In this way, controls the DSB repair pathway, pairing of homologous chromosomes and sex body formation (By similarity).
Moreover plays a central role in the transcriptional activation of genes during early meiotic prophase thanks to H3K4me3 and H3K36me3 enrichment that represents a specific tag for epigenetic transcriptional activation (By similarity).
In addition performs automethylation (By similarity).
Acetylation and phosphorylation of histone H3 attenuate or prevent histone H3 methylation (By similarity).
Biological Process
Chromatin organizationIEA:UniProtKB-KW
Double-strand break repair involved in meiotic recombinationISS:UniProtKB
Female gamete generationISS:UniProtKB
Histone H3-K36 dimethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K36 trimethylationISS:UniProtKB
Histone H3-K4 dimethylationISS:UniProtKB
Histone H3-K4 methylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K4 monomethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K4 trimethylationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-K9 methylationISS:UniProtKB
Homologous chromosome pairing at meiosisISS:UniProtKB
Male gamete generationISS:UniProtKB
Meiotic gene conversionManual Assertion Based On ExperimentIDA:MGI
Negative regulation of apoptotic processISS:UniProtKB
Positive regulation of fertilizationISS:UniProtKB
Positive regulation of histone H3-K36 trimethylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of histone H3-K4 trimethylationISS:UniProtKB
Positive regulation of reciprocal meiotic recombinationManual Assertion Based On ExperimentIMP:MGI
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of transcription, DNA-templatedIEA:InterPro
Cellular Location
Nucleus
Chromosome
Localizes in nuclei of pre-leptotene, leptotene, and early to mid-zygotene spermatocytes.
PTM
Mono-methylated; automethylated. Tri-methylated; automethylated. Mono-methylation is predominant; automethylation is lower and slower than H3 peptide methylation and is in a highest S-adenosyl-L-methionine concentration-dependent. There are two major sites for automethylation at Lys-368 and Lys-374. Lysines can be simultaneously methylated, such as Lys-368(me3)/Lys-372(me1), Lys-368(me1)/Lys-374(me1) and Lys-368(me1)/Lys-372(me1)/Lys-374(me1). Automethylation is an intramolecular (cis) process.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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