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Mouse Anti-PRIM2 Recombinant Antibody (4G2) (CBMAB-P2777-YC)

Provided herein is a Mouse monoclonal antibody against Human DNA Primase Subunit 2. The antibody can be used for immunoassay techniques, such as ELISA.
See all PRIM2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4G2
Antibody Isotype
IgG2b, κ
Application
ELISA

Basic Information

Immunogen
PRIM2A (AAH17833, AA 1-158 full length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Primase Subunit 2
Introduction
PRIM2 is the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA.
Entrez Gene ID
5558
UniProt ID
P49643
Alternative Names
PRIM2A; p58
Function
Regulatory subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis (PubMed:9705292, PubMed:17893144, PubMed:25550159, PubMed:26975377).
During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity).
The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (PubMed:17893144).
These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).
In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (PubMed:17893144, PubMed:25550159).
Binds RNA:DNA duplex and coordinates the catalytic activities of PRIM1 and POLA2 during primase-to-polymerase switch.
Biological Process
DNA replication initiationManual Assertion Based On ExperimentIDA:ComplexPortal
DNA replication, synthesis of RNA primerManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of DNA primase activityManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
alpha DNA polymerase:primase complex
nucleoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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