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Recombinant Mouse Anti-PRKAB1 Antibody (CBAb196) (CBMAB-Ab196-LY)

The product is antibody recognizes PRKAB1. The antibody CBAb196 immunoassay techniques such as: WB 1:500-1:2000; IHC 1:50-1:200; ICC 1:20-1:50; IP 1:50-1:200.
See all PRKAB1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Monkey
Clone
CBAb196
Antibody Isotype
IgG
Application
WB 1:500-1:2000; IHC 1:50-1:200; ICC 1:20-1:50; IP 1:50-1:200

Basic Information

Immunogen
Recombinant protein of human PRKAB1
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
PRKAB1
Introduction
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
Entrez Gene ID
Human5564
Mouse19079
Rat83803
Monkey695737
UniProt ID
HumanQ9Y478
MouseQ9R078
RatP80386
MonkeyH9EPL6
Alternative Names
PRKAB1; AMPK; HAMPKb; 5'-AMP-activated protein kinase subunit beta-1
Function
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Biological Process
Cellular response to nutrient levelsManual Assertion Based On ExperimentIDA:ComplexPortal
Fatty acid biosynthetic processIEA:UniProtKB-KW
Nail developmentIEA:Ensembl
Positive regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Positive regulation of gene expressionManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of catalytic activityIEA:InterPro
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
cytoplasm
cytosol
nucleoplasm
nucleotide-activated protein kinase complex
nucleus
PTM
Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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