Rabbit Anti-PRKACA Recombinant Antibody (D38C6) (CBMAB-P2796-YC)

Rabbit

Human, Hamster, Monkey, Mouse, Rat

D38C6

IgG

IP, WB

Synthetic peptide corresponding to residues surrounding Ser326 of human PKA C-a

Human, Hamster, Monkey, Mouse, Rat

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid in 10 mM, pH 7.5, 100 µg/ml BSA, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

protein kinase, cAMP-dependent, catalytic, alpha

PRKACA is one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis.

PKACA; PPNAD4

Phosphorylates a large number of substrates in the cytoplasm and the nucleus (PubMed:15642694, PubMed:15905176, PubMed:16387847, PubMed:17333334, PubMed:17565987, PubMed:17693412, PubMed:18836454, PubMed:19949837, PubMed:20356841, PubMed:21085490, PubMed:21514275, PubMed:21812984).
Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP (PubMed:15642694, PubMed:15905176, PubMed:16387847, PubMed:17333334, PubMed:17565987, PubMed:17693412, PubMed:18836454, PubMed:19949837, PubMed:20356841, PubMed:21085490, PubMed:21514275, PubMed:21812984).
Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed:21423175).
RORA is activated by phosphorylation (PubMed:21514275).
Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts (PubMed:19949837).
Involved in chondrogenesis by mediating phosphorylation of SOX9 (By similarity).
Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP (PubMed:15642694, PubMed:20356841).
Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated (PubMed:17333334).
RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca2+, leading to reduced amplitude and increased frequency of store overload-induced Ca2+ release (SOICR) characterized by an increased rate of Ca2+ release and propagation velocity of spontaneous Ca2+ waves, despite reduced wave amplitude and resting cytosolic Ca2+ (PubMed:17693412).
PSMC5/RPT6 activation by phosphorylation stimulates proteasome (PubMed:17565987).
Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation (PubMed:15905176).
NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding (PubMed:15642694).
Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation (By similarity).
May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) (By similarity).
Phosphorylates APOBEC3G and AICDA (PubMed:16387847, PubMed:18836454).
Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed:21085490).
Isoform 2
Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.

Calcium-mediated signaling using intracellular calcium sourceManual Assertion Based On ExperimentTAS:BHF-UCL
Cell communication by electrical coupling involved in cardiac conductionManual Assertion Based On ExperimentTAS:BHF-UCL
Cellular response to coldIEA:Ensembl
Cellular response to epinephrine stimulusManual Assertion Based On ExperimentTAS:BHF-UCL
Cellular response to glucose stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to heatManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to parathyroid hormone stimulusIEA:Ensembl
Cytokine-mediated signaling pathwayTAS:Reactome
High-density lipoprotein particle assemblyTAS:Reactome
Mesoderm formationIEA:Ensembl
Modulation of chemical synaptic transmissionIEA:Ensembl
mRNA processingManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningIEA:Ensembl
Neural tube closureIEA:Ensembl
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
Peptidyl-threonine phosphorylationIEA:Ensembl
Positive regulation of protein export from nucleusIEA:Ensembl
Protein autophosphorylationIEA:Ensembl
Protein kinase A signalingManual Assertion Based On ExperimentIBA:GO_Central
Protein localization to lipid dropletIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of bicellular tight junction assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cardiac conductionTAS:Reactome
Regulation of cardiac muscle contractionManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of cell cycleISS:UniProtKB
Regulation of cytosolic calcium ion concentrationManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of heart rateManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of macroautophagyManual Assertion Based On ExperimentTAS:ParkinsonsUK-UCL
Regulation of osteoblast differentiationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of proteasomal protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of protein bindingManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of protein processingIEA:Ensembl
Regulation of ryanodine-sensitive calcium-release channel activityManual Assertion Based On ExperimentTAS:BHF-UCL
Renal water homeostasisTAS:Reactome
Sperm capacitationISS:UniProtKB

Cytoplasm
Cell membrane
Nucleus
Mitochondrion
Membrane
Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity).
Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (PubMed:21085490).
Isoform 2
Cell projection, cilium, flagellum
Cytoplasmic vesicle, secretory vesicle, acrosome
Expressed in the midpiece region of the sperm flagellum (PubMed:10906071).
Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardless of the capacitation status of the sperm (By similarity).

Primary pigmented nodular adrenocortical disease 4 (PPNAD4):
A rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. Adrenal glands show overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. Clinical manifestations of Cushing syndrome include facial and truncal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes.
Cardioacrofacial dysplasia 1 (CAFD1):
An autosomal dominant disease characterized by dysmorphic facial features, congenital cardiac defects, primarily common atrium or atrioventricular septal defect, and limb anomalies, including short limbs, brachydactyly and postaxial polydactyly.

Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
Autophosphorylated. Phosphorylation is enhanced by vitamin K2. Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity.
Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficiency.