Rabbit Anti-PRKCB Recombinant Antibody (D3E7O) (CBMAB-CP1985-LY)

Rabbit

Human, Mouse, Rat

D3E7O

IgG

WB, IP, FC

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro619 of human PKCβI protein.

Human, Mouse, Rat

IgG

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

100 µg/ml BSA, 50% glycerol

0.02% sodium azide

> 95% Purity determined by SDS-PAGE.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Protein Kinase C Beta

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity (PubMed:11598012).
Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A (PubMed:20228790).
In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1 (PubMed:25982116).
Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity).
Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription (PubMed:19176525).

Adaptive immune responseIEA:UniProtKB-KW
Apoptotic processIEA:UniProtKB-KW
B cell activationISS:UniProtKB
B cell receptor signaling pathwayISS:UniProtKB
Calcium ion transportIEA:Ensembl
Cellular calcium ion homeostasisIEA:Ensembl
Cellular response to carbohydrate stimulusIEA:Ensembl
Chromatin organizationIEA:UniProtKB-KW
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Lipoprotein transportManual Assertion Based On ExperimentTAS:BHF-UCL
Mitotic nuclear membrane disassemblyTAS:Reactome
Negative regulation of glucose transmembrane transportISS:UniProtKB
Negative regulation of insulin receptor signaling pathwayISS:UniProtKB
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of angiogenesisISS:UniProtKB
Positive regulation of B cell receptor signaling pathwayISS:UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signalingISS:UniProtKB
Positive regulation of NF-kappaB transcription factor activityISS:UniProtKB
Positive regulation of vascular endothelial growth factor receptor signaling pathwayISS:UniProtKB
Post-translational protein modificationManual Assertion Based On ExperimentIDA:UniProtKB
Presynaptic modulation of chemical synaptic transmissionIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
Regulation of glucose transmembrane transportManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of synaptic vesicle exocytosisIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:UniProtKB
Signal transduction1 PublicationNAS:ProtInc

Cytoplasm
Nucleus
Membrane

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).