Rabbit Anti-PRKCZ Recombinant Antibody (R14-K) (CBMAB-1707-CN)

PRKCZ

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro (PubMed:17313651).

PKC2; PKC-ZETA

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed:17313651).
Isoform 2
Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.

Activation of phospholipase D activityIEA:Ensembl
Activation of protein kinase B activityIEA:Ensembl
Cell migrationIEA:Ensembl
Cell surface receptor signaling pathwayIEA:Ensembl
Cellular response to insulin stimulusIEA:Ensembl
Establishment of cell polarityISS:UniProtKB
Inflammatory responseIEA:UniProtKB-KW
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Long-term memoryIEA:Ensembl
Long-term synaptic potentiationISS:UniProtKB
Membrane depolarizationIEA:Ensembl
Membrane hyperpolarizationIEA:Ensembl
Microtubule cytoskeleton organizationIEA:Ensembl
Negative regulation of apoptotic processManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of hydrolase activityIEA:Ensembl
Negative regulation of insulin receptor signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of peptidyl-tyrosine phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of protein-containing complex assemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Neuron projection extensionIEA:Ensembl
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of cell population proliferationIEA:Ensembl
Positive regulation of cell-matrix adhesionIEA:Ensembl
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of excitatory postsynaptic potentialISS:UniProtKB
Positive regulation of insulin receptor signaling pathwayISS:UniProtKB
Positive regulation of interleukin-10 productionISS:UniProtKB
Positive regulation of interleukin-13 productionISS:UniProtKB
Positive regulation of interleukin-4 productionISS:UniProtKB
Positive regulation of interleukin-5 productionISS:UniProtKB
Positive regulation of NF-kappaB transcription factor activityISS:UniProtKB
Positive regulation of protein transportIEA:Ensembl
Positive regulation of T-helper 2 cell cytokine productionISS:UniProtKB
Positive regulation of T-helper 2 cell differentiationISS:UniProtKB
Protein kinase C signalingIEA:Ensembl
Protein localization to plasma membraneIEA:Ensembl
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of neurotransmitter receptor localization to postsynaptic specialization membraneIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Vesicle transport along microtubuleIEA:Ensembl

Cytoplasm
Endosome
Cell junction
Membrane
In the retina, localizes in the terminals of the rod bipolar cells (By similarity).
Associates with endosomes (PubMed:9566925).
Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (PubMed:7597083).
Colocalizes with VAMP2 and WDFY2 in intracellular vesicles (PubMed:17313651).
Transiently translocates to the membrane of CA1 hippocampal cells in response to the induction of long term potentiation (By similarity).
Isoform 2
Cytoplasm

CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.