Mouse Anti-PRKRA Monoclonal Antibody (3A4) (CBMAB-0007-LY)

Name: Mouse Anti-PRKRA Monoclonal Antibody (3A4)
SKU: CBMAB-0007-LY
Rating: 5 (1 reviews)

See all PRKRA antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3A4

Application

IP, MA

Immunogen

Recombinant peptide (Full Length)

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

PRKRA

Introduction

This gene encodes a protein that belongs to a conserved family of histone methyltransferases that predominantly act as negative regulators of transcription. The encoded protein contains an N-terminal Su(var)3-9, Enhancer-of-zeste, and Trithorax (SET) domain and a double zinc-finger domain. The protein activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis.

Entrez Gene ID

8575

UniProt ID

O75569

Alternative Names

DYT16; HSD14; PACT; RAX

Function

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).

Biological Process

Cellular response to oxidative stressIEA:Ensembl
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Middle ear morphogenesisIEA:Ensembl
miRNA processingManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Outer ear morphogenesisIEA:Ensembl
Positive regulation of intrinsic apoptotic signaling pathwayIEA:Ensembl
Pre-miRNA processingManual Assertion Based On ExperimentIDA:BHF-UCL
Protein phosphorylationIEA:Ensembl
Protein stabilizationManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of production of small RNA involved in gene silencing by RNAManual Assertion Based On ExperimentIBA:GO_Central
Response to virusManual Assertion Based On ExperimentTAS:ProtInc
siRNA processingManual Assertion Based On ExperimentIDA:UniProtKB
Skeletal system morphogenesisIEA:Ensembl

Cellular Location

Cytoplasm, perinuclear region
Cytoplasm

Involvement in disease

Dystonia 16 (DYT16):
An early-onset dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT16 patients have progressive, generalized dystonia with axial muscle involvement, oro-mandibular (sardonic smile) and laryngeal dystonia and, in some cases, parkinsonian features.

PTM

Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-PRKRA Monoclonal Antibody (3A4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

PRKRA Matched Antibody Pair (930) (CAT#: APMAB-930LY)

Related Products

Mouse Anti-PRKRA Recombinant Antibody (2830C1a) (CAT#: CBMAB-P2891-YC)

PRKRA Matched Antibody Pair (930) (CAT#: APMAB-930LY)

Rabbit Anti-PRKRA Recombinant Antibody (D9N6J) (CAT#: CBMAB-P2892-YC)

Mouse Anti-PRKRA Recombinant Antibody (1G12) (CAT#: CBMAB-A7061-LY)

Rabbit Anti-PRKRA Recombinant Antibody (D9N6J) (CAT#: CBMAB-CP1853-LY)

Mouse Anti-PRKRA Recombinant Antibody (1B9-1A7) (CAT#: CBMAB-P2890-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry