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Mouse Anti-PUF60 Recombinant Antibody (917) (CBMAB-P3512-YC)

Provided herein is a Mouse monoclonal antibody against Human Poly-U binding splicing factor 60. The antibody can be used for immunoassay techniques, such as WB.
See all PUF60 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
917
Antibody Isotype
IgG2b
Application
WB

Basic Information

Immunogen
Recombinant protein encompassing a sequence within the center region of human PUF60.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
poly-U binding splicing factor 60KDa
Introduction
PUF60 is a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor.
Entrez Gene ID
Human22827
Mouse67959
Rat84401
UniProt ID
HumanQ9UHX1
MouseQ3UEB3
RatQ9WV25
Alternative Names
2410104I19Rik; 2810454F19Rik; SIAHBP1
Function
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA.
Biological Process
Alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Apoptotic processIEA:UniProtKB-KW
mRNA splice site selectionManual Assertion Based On ExperimentIBA:GO_Central
Regulation of alternative mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Colocalizes partially with RO60.
Involvement in disease
Verheij syndrome (VRJS):
A syndrome characterized by growth retardation, delayed psychomotor development, dysmorphic facial features, and skeletal, mainly vertebral, abnormalities. Additional variable features may include coloboma, renal defects, and cardiac defects.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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