Mouse Anti-PUS1 Recombinant Antibody (CBYC-P802) (CBMAB-P3531-YC)

Name: Mouse Anti-PUS1 Recombinant Antibody (CBYC-P802)
SKU: CBMAB-P3531-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

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Basic Information

Host Animal

Mouse

Clone

CBYC-P802

Application

IF, IP, WB, ELISA

Immunogen

PUS1 (AAH02901, 1-399 aa) full length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

pseudouridylate synthase 1

Introduction

PUS1 is a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia.

Entrez Gene ID

80324

UniProt ID

Q9Y606

Alternative Names

MLASA1

Function

Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs (PubMed:15772074, PubMed:24722331).
Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed:24722331).
Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:31477916, PubMed:35051350).
Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (PubMed:35051350).
Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (PubMed:35051350).
Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA (PubMed:24722331).

Biological Process

Mitochondrial tRNA pseudouridine synthesisTAS:Reactome
mRNA processingIEA:UniProtKB-KW
mRNA pseudouridine synthesisManual Assertion Based On ExperimentIDA:UniProtKB
RNA splicingIEA:UniProtKB-KW
tRNA pseudouridine synthesisManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Isoform 1
Mitochondrion
Isoform 2
Nucleus
Cytoplasm

Involvement in disease

Myopathy with lactic acidosis and sideroblastic anemia 1 (MLASA1):
A rare oxidative phosphorylation disorder specific to skeletal muscle and bone marrow. Affected individuals manifest progressive muscle weakness, exercise intolerance, lactic acidosis, sideroblastic anemia and delayed growth.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-PUS1 Recombinant Antibody (A-4) (CAT#: CBMAB-P3529-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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