Rabbit Anti-QTRT1 Recombinant Antibody (RB37610) (CBMAB-01936-YC)

Name: Rabbit Anti-QTRT1 Recombinant Antibody (RB37610)
SKU: CBMAB-01936-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

RB37610

Application

ELISA, WB

Immunogen

A KLH conjugated synthetic peptide between 345-371 amino acids from the C-terminal region of human QTRT1

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

Localized in a.a.345-371

More Infomation

Target

Full Name

Queuine TRNA-Ribosyltransferase Catalytic Subunit 1

Introduction

QTRT1 is the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine.

Entrez Gene ID

81890

UniProt ID

Q9BXR0

Alternative Names

TGT; TGUT; FP3235

Function

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154).
Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).

Biological Process

Biological Process tRNA modificationManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process tRNA-guanine transglycosylationManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm
Mitochondrion outer membrane
Weakly associates with mitochondria, possibly via QTRT2.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-QTRT1 Recombinant Antibody (D-7) (CAT#: CBMAB-1859-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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