Sign in or Register   Sign in or Register
  |  

Mouse Anti-RAB1B Recombinant Antibody (1B2) (CBMAB-R0734-CN)

This product is a Mouse antibody that recognizes RAB1B. The antibody 1B2 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all RAB1B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1B2
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa. 91-202 from human RAB1B (NP_112243) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2

Target

Full Name
RAB1B, member RAS oncogene family
Introduction
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]). [supplied by OMIM, Jan 2009]
Entrez Gene ID
81876
UniProt ID
Q9H0U4
Alternative Names
RAB1B, Member RAS Oncogene Family;
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:20545908, PubMed:9437002).
Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:9437002).
Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum (PubMed:20545908).
Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments (By similarity).
Required to modulate the compacted morphology of the Golgi (PubMed:26209634).
Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment (By similarity).
Biological Process
Biological Process autophagosome assemblyManual Assertion Based On ExperimentIBA:GO_Central
Biological Process endoplasmic reticulum to Golgi vesicle-mediated transportManual Assertion Based On ExperimentIGI:UniProtKB
Biological Process Golgi organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process intracellular protein transportManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of glycoprotein metabolic processManual Assertion Based On ExperimentIGI:UniProtKB
Biological Process regulation of autophagosome assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process virion assemblyManual Assertion Based On ExperimentIGI:UniProtKB
Cellular Location
Cytoplasm
Membrane
Preautophagosomal structure membrane
Cytoplasm, perinuclear region
Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments (PubMed:11389151).
In the GDP-form, colocalizes with GDI in the cytoplasm (PubMed:11389151).
Co-localizes with MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment and to the peri-Golgi region (By similarity).
PTM
Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
(Microbial infection) AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. It is later de-AMPylated by L.pneumophila SidD, releasing RAB1B from bacterial phagosomes.
(Microbial infection) Phosphocholinated at Ser-76 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI) (PubMed:21822290, PubMed:22307087).
Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB (PubMed:22158903, PubMed:22307087).
(Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-RAB1B Recombinant Antibody (1B2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare