Mouse Anti-RAD54L Recombinant Antibody (60.1) (CBMAB-R1144-CN)

Name: Mouse Anti-RAD54L Recombinant Antibody (60.1)
SKU: CBMAB-R1144-CN
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

60.1

Application

ELISA, WB, IHC

Immunogen

RAD54L monoclonal antibody was raised against a combination of N & C peptide, corresponding to a range between aa. 1-300 and 450-741

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

More Infomation

Target

Full Name

RAD54-like (S. cerevisiae)

Introduction

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Dec 2008]

Entrez Gene ID

8438

UniProt ID

Q92698

Alternative Names

RAD54 Like; RAD54 Homolog; RAD54A; HRAD54; HHR54; DNA Repair And Recombination Protein RAD54-Like;

Function

Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks (PubMed:9774452, PubMed:24798879, PubMed:32457312, PubMed:11459989, PubMed:12205100, PubMed:27264870).
Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (By similarity).

Biological Process

Biological Process determination of adult lifespanIEA:Ensembl
Biological Process DNA recombinationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Biological Process double-strand break repair via synthesis-dependent strand annealingManual Assertion Based On ExperimentIBA:GO_Central
Biological Process meiotic cell cycleManual Assertion Based On ExperimentTAS:ProtInc
Biological Process reciprocal meiotic recombinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process response to ionizing radiationIEA:Ensembl
Biological Process response to xenobiotic stimulusIEA:Ensembl

Cellular Location

Nucleus

PTM

Acetylated. Acetylation promotes interaction with BRD9, and subsequently with RAD54, which is essential for homologous recombination (HR).
Phosphorylated. Phosphorylation at Ser-572 by NEK1 specifically in G2 phase allows efficient removal of RAD51 filaments from DNA.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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