Rabbit Anti-RBL1 Recombinant Antibody (D3P3C) (CBMAB-CP2224-LY)

Name: Rabbit Anti-RBL1 Recombinant Antibody (D3P3C)
SKU: CBMAB-CP2224-LY
Rating: 5 (1 reviews)

See all RBL1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D3P3C

Application

WB, IP

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro341 of human RBL1 protein.

Specificity

Human, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

RB Transcriptional Corepressor Like 1

Introduction

The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	5933
Monkey	701650

UniProt ID

Human	P28749
Monkey	F7CLV0

Alternative Names

RB Transcriptional Corepressor Like 1; 107 KDa Retinoblastoma-Associated Protein; Retinoblastoma-Like 1; PRB1; P107; Retinoblastoma-Like Protein 1; CP107;

Function

Key regulator of entry into cell division (PubMed:17671431).
Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation (By similarity).
Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression (By similarity).
Controls histone H4 'Lys-20' trimethylation (By similarity).
Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters (By similarity).
Potent inhibitor of E2F-mediated trans-activation (PubMed:8319904).
May act as a tumor suppressor (PubMed:8319904).

Biological Process

Biological Process cell cycleIEA:UniProtKB-KW
Biological Process cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process chromatin organizationIEA:UniProtKB-KW
Biological Process negative regulation of cellular senescenceManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of gene expressionManual Assertion Based On ExperimentIMP:MGI
Biological Process negative regulation of transcription by RNA polymerase IIIEA:Ensembl
Biological Process regulation of cell divisionIEA:InterPro
Biological Process regulation of lipid kinase activityManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus

PTM

Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-RBL1 Recombinant Antibody (D3P3C)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-RBL1 Recombinant Antibody (1A5) (CAT#: CBMAB-R1698-CN)

Mouse Anti-RBL1 Recombinant Antibody (KAB6) (CAT#: CBMAB-R1699-CN)

Mouse Anti-RBL1 Recombinant Antibody (SD9) (CAT#: CBMAB-P3598-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry