Rabbit Anti-RGS6 Recombinant Antibody (
12H5) (V2LY-0725-LY1118)

Name: Rabbit Anti-RGS6 Recombinant Antibody (12H5)
SKU: V2LY-0725-LY1118
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-RGS6 Recombinant Antibody (12H5) (V2LY-0725-LY1118) in IHC

IHC image of V2LY-0725-LY1118 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-RGS6 Recombinant Antibody (12H5) (V2LY-0725-LY1118) in IHC

IHC image of V2LY-0725-LY1118 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-RGS6 Recombinant Antibody (12H5) (V2LY-0725-LY1118) in IF

Immunofluorescence staining of PC-3 cell with V2LY-0725-LY1118 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 592-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

12H5

Application

ELISA, IHC, IF

Immunogen

A synthesized peptide derived from human RGS6.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Regulator Of G Protein Signaling 6

Entrez Gene ID

9628

UniProt ID

P49758

Function

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity (PubMed:10521509).

Biological Process

Biological Process G protein-coupled receptor signaling pathwayTAS:Reactome
Biological Process intracellular signal transductionIEA:InterPro
Biological Process negative regulation of signal transductionIEA:UniProtKB-KW
Biological Process positive regulation of GTPase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Cytoplasm, cytosol
Membrane
Nucleus
Cell membrane
Interaction with GNB5 mediates translocation to the nucleus.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-RGS6 Recombinant Antibody (CBLG1-2432) (CAT#: CBMAB-G6532-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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