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Mouse Anti-RNASEH1 Recombinant Antibody (5D10) (CBMAB-A7624-LY)

The product is antibody recognizes RNASEH1. The antibody 5D10 immunoassay techniques such as: WB, ELISA.
See all RNASEH1 antibodies
Published Data
Fig.1 Western analysis of RNH1 expression in siCtrl and RNH1-depleted 293T cells (siRNH1).
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Fig.2 Representative metaphase chromosomes processed with CO-FISH from RPE1 cells expressing depleted of FEN1 (shFEN1), with or without ectopically expressed RNase H1 (Ad-RH1).
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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
5D10
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
RNASEH1 (NP_002927, 189 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ribonuclease H1
Entrez Gene ID
Human246243
Rat298933
UniProt ID
HumanO60930
RatQ5BK46
Alternative Names
H1RNA
Function
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (PubMed:10497183).
Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II (PubMed:21700224).
Biological Process
Biological Process DNA replication, removal of RNA primerManual Assertion Based On ExperimentIBA:GO_Central
Biological Process RNA catabolic processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process RNA phosphodiester bond hydrolysis, endonucleolyticManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Involvement in disease
Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2 (PEOB2):
A form of progressive external ophthalmoplegia, a mitochondrial myopathy characterized by progressive paralysis of the levator palpebrae, orbicularis oculi, and extraocular muscles. PEOB2 patients manifest exercise intolerance, muscle weakness, and signs and symptoms of spinocerebellar ataxia, such as impaired gait and dysarthria. Some patients may have respiratory insufficiency.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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