Mouse Anti-RNF125 Recombinant Antibody (CBYJT-1642) (CBMAB-T0688-YJ)

Name: Mouse Anti-RNF125 Recombinant Antibody (CBYJT-1642)
SKU: CBMAB-T0688-YJ
Rating: 5 (1 reviews)

See all RNF125 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-1642

Application

ELISA, ICC, IF, WB

Immunogen

RNF125 (NP_060301, 143 a.a. ~ 231 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 143-231

More Infomation

Target

Full Name

ring finger protein 125

Introduction

RNF125 is a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, RNF125 associates with membranes and is primarily localized to intracellular membrane systems. RNF125 may play a role as a positive regulator in the T-cell receptor signaling pathway.

Entrez Gene ID

54941

UniProt ID

Q96EQ8

Alternative Names

Ring Finger Protein 125; Ring Finger Protein 125, E3 Ubiquitin Protein Ligase; T-Cell RING Activation Protein 1; RING Finger Protein 125; TRAC-1; T-Cell Ring Protein Identified In Activation Screen

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as DDX58/RIG-I, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed:15843525, PubMed:17460044, PubMed:17643463, PubMed:26027934, PubMed:26471729, PubMed:25591766, PubMed:27411375).
Acts as a negative regulator of type I interferon production by mediating ubiquitination of DDX58/RIG-I at 'Lys-181', leading to DDX58/RIG-I degradation (PubMed:17460044, PubMed:26471729).
Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed:25591766).
Mediates ubiquitination and subsequent degradation of JAK1 (PubMed:26027934).
Acts as a positive regulator of T-cell activation (PubMed:15843525).

Biological Process

Biological Process adaptive immune responseIEA:UniProtKB-KW
Biological Process cellular response to leukemia inhibitory factorIEA:Ensembl
Biological Process negative regulation of RIG-I signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of type I interferon productionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Golgi apparatus membrane
Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane.

Involvement in disease

Tenorio syndrome (TNORS):
A disease characterized by overgrowth, macrocephaly, and intellectual disability. Some patients may have mild hydrocephaly, hypoglycemia, and inflammatory diseases resembling Sjogren syndrome.

PTM

Autoubiquitinated, leading to its subsequent proteasomal degradation.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-RNF125 Recombinant Antibody (CBYJT-1642)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-RNF125 Recombinant Antibody (1A5) (CAT#: CBMAB-R2911-CN)

Mouse Anti-RNF125 Recombinant Antibody (CBCNR-572) (CAT#: CBMAB-R2913-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry