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Mouse Anti-RNF125 Recombinant Antibody (CBYJT-1642) (CBMAB-T0688-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to RNF125 (Ring Finger Protein 125). The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, WB.
See all RNF125 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-1642
Antibody Isotype
IgG1, κ
Application
ELISA, ICC, IF, WB

Basic Information

Immunogen
RNF125 (NP_060301, 143 a.a. ~ 231 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 143-231

Target

Full Name
ring finger protein 125
Introduction
RNF125 is a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, RNF125 associates with membranes and is primarily localized to intracellular membrane systems. RNF125 may play a role as a positive regulator in the T-cell receptor signaling pathway.
Entrez Gene ID
54941
UniProt ID
Q96EQ8
Alternative Names
Ring Finger Protein 125; Ring Finger Protein 125, E3 Ubiquitin Protein Ligase; T-Cell RING Activation Protein 1; RING Finger Protein 125; TRAC-1; T-Cell Ring Protein Identified In Activation Screen
Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as DDX58/RIG-I, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed:15843525, PubMed:17460044, PubMed:17643463, PubMed:26027934, PubMed:26471729, PubMed:25591766, PubMed:27411375).
Acts as a negative regulator of type I interferon production by mediating ubiquitination of DDX58/RIG-I at 'Lys-181', leading to DDX58/RIG-I degradation (PubMed:17460044, PubMed:26471729).
Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed:25591766).
Mediates ubiquitination and subsequent degradation of JAK1 (PubMed:26027934).
Acts as a positive regulator of T-cell activation (PubMed:15843525).
Biological Process
Biological Process adaptive immune responseIEA:UniProtKB-KW
Biological Process cellular response to leukemia inhibitory factorIEA:Ensembl
Biological Process negative regulation of RIG-I signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of type I interferon productionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Golgi apparatus membrane
Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane.
Involvement in disease
Tenorio syndrome (TNORS):
A disease characterized by overgrowth, macrocephaly, and intellectual disability. Some patients may have mild hydrocephaly, hypoglycemia, and inflammatory diseases resembling Sjogren syndrome.
PTM
Autoubiquitinated, leading to its subsequent proteasomal degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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