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Mouse Anti-RNF128 Recombinant Antibody (CBLG1-3244) (CBMAB-G6533-LY)

This product is antibody recognizes RNF128. The antibody CBLG1-3244 immunoassay techniques such as: WB.
See all RNF128 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLG1-3244
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ring Finger Protein 128, E3 Ubiquitin Protein Ligase
Introduction
The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Entrez Gene ID
79589
UniProt ID
Q8TEB7
Alternative Names
Ring Finger Protein 128; E3 Ubiquitin Protein Ligase; Gene Related To Anergy In Lymphocytes Protein; RING-Type E3 Ubiquitin Transferase RNF128; GRAIL; E3 Ubiquitin-Protein Ligase RNF128; Ring Finger Protein 128; RING Finger Protein 128; EC 2.3.2.27; EC 6.3.2;
Function
E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
Biological Process
Biological Process negative regulation of cytokine productionIEA:Ensembl
Biological Process positive regulation of protein catabolic process in the vacuole1 PublicationIC:BHF-UCL
Biological Process protein localization to lysosome1 PublicationIC:BHF-UCL
Biological Process regulation of protein stabilityManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Endomembrane system
Cytoplasm, cytoskeleton
Cytoplasm, perinuclear region
Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity).
Topology
Helical: 208-228
PTM
Auto-ubiquitinated. Controls the development of T-cell clonal anergy by ubiquitination.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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