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Mouse Anti-RNF139 Recombinant Antibody (3D10) (CBMAB-R2924-CN)

This product is a Mouse antibody that recognizes RNF139. The antibody 3D10 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all RNF139 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3D10
Antibody Isotype
IgG1, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa. 565-665 from RNF139 (NP_009149) with GST tag
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2

Target

Full Name
ring finger protein 139
Introduction
The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008]
Entrez Gene ID
11236
UniProt ID
Q8WU17
Alternative Names
Ring Finger Protein 139; Translocation In Renal Carcinoma On Chromosome 8 Protein; RING-Type E3 Ubiquitin Transferase RNF139; RING Finger Protein 139; TRC8; Patched Related Protein Translocated In Renal Cancer;
Function
E3-ubiquitin ligase; acts as a negative regulator of cell proliferation through mechanisms involving G2/M arrest and cell death (PubMed:10500182, PubMed:12032852, PubMed:17016439).
Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER) (PubMed:19720873).
Affects SREBP processing by hindering the SREBP-SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression (PubMed:19706601, PubMed:20068067).
Involved in the sterol-accelerated degradation of HMGCR (PubMed:22143767, PubMed:23223569).
This is achieved through binding of RNF139 to INSIG1 and/or INSIG2 at the ER membrane (PubMed:22143767).
In addition, interaction of RNF139 with AUP1 facilitates interaction of RNF139 with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase AMFR, leading to ubiquitination of HMGCR (PubMed:23223569).
The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction (PubMed:22143767, PubMed:23223569).
Required for INSIG1 ubiquitination (PubMed:20068067).
May be required for EIF3 complex ubiquitination (PubMed:20068067).
Biological Process
Biological Process endoplasmic reticulum mannose trimmingTAS:Reactome
Biological Process ERAD pathwayManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Biological Process negative regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of translationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein destabilizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of ER to Golgi vesicle-mediated transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of protein processingManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Endoplasmic reticulum membrane
Involvement in disease
Renal cell carcinoma (RCC):
Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Clear cell renal cell carcinoma is the most common subtype.
PTM
Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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