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Mouse Anti-RNF187 Recombinant Antibody (5H10) (CBMAB-R2980-CN)

This product is a Mouse antibody that recognizes RNF187. The antibody 5H10 can be used for immunoassay techniques such as: WB, IP, ELISA.
See all RNF187 antibodies
Published Data
Fig.1 Confirmation that endogenous RACO‑1 is a protein with a relative molecular mass of 26K. Silencing endogenous RACO‑1 in PC12 cells results in the disappearance of the RACO‑1 immunoreactive band.
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Fig.2 RACO‑1 is required for effective cell-cycle progression. NIH3T3 shLUC or NIH3T3 shRACO-1a cells were serum-starved for 24 h before the addition of 10% FCS and BrdU for the time points indicated. Cell-cycle profiles were determined by propidium iodide incorporation and flow cytometry analysis (mean ± s.d, n = 4).
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Fig.3 RACO‑1 cooperated with oncogenic K‑Ras, inducing colonic epithelial hyperplasia in vivo.
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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
5H10
Antibody Isotype
IgG1
Application
WB, IP, ELISA

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
1 mg/mL

Target

Full Name
Ring Finger Protein 187
Introduction
RNF187 (Ring Finger Protein 187) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin-protein transferase activity.
Entrez Gene ID
Human149603
Mouse108660
Rat360533
UniProt ID
HumanQ5TA31
MouseQ8BFX1
RatD3Z8N2
Alternative Names
Ring Finger Protein 187; RING-Type E3 Ubiquitin Transferase RNF187; Ring Domain AP-1 Co-Activator 1; RING Domain AP1 Coactivator 1; RING Finger Protein 187; RACO-1;
Function
E3 ubiquitin-protein ligase that acts as a coactivator of JUN-mediated gene activation in response to growth factor signaling via the MAP3K1 pathway, independently from MAPK8.
Biological Process
Biological Process innate immune responseManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of cell population proliferationISS:UniProtKB
Biological Process positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein autoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein K48-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Nucleus
Predominantly located in the cytoplasm. Shuttles between the cytoplasm and the nucleus.
PTM
Ubiquitinated; undergoes 'Lys-48'-linked autoubiquitination in the absence of growth factors and MAP3K1-induced 'Lys-63'-linked polyubiquitination (PubMed:20852630).
'Lys-48'-autoubiquitination leads to degradation by the proteasome, while MAP3K1-induced 'Lys-63'-linked polyubiquitination results in the stabilization of the protein (PubMed:20852630).
'Lys-48'- and 'Lys-63'-linked polyubiquitinations occur most probably on the same 3 C-terminal lysine residues (Lys-195, Lys-223 and Lys-224) and are thus mutually exclusive (PubMed:20852630).
Other sites of ubiquitination are not excluded (PubMed:20852630).
'Lys-63'-linked polyubiquitination by TRIM7 in response to growth factor signaling via the MEK/ERK pathway enhances protein stability (PubMed:25851810).
Arginine methylation by PRMT1 stabilizes RNF187 by facilitating K63-linked ubiquitin chain formation, and enables dimerization, c-Jun interaction and subsequent AP1 target gene expression.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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