Mouse Anti-RNF20 Monoclonal Antibody (1D7) (CBMAB-0055-LY)

Name: Mouse Anti-RNF20 Monoclonal Antibody (1D7)
SKU: CBMAB-0055-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D7

Application

MA, WB

Immunogen

Recombinant peptide (Protein Domain)

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

RNF20

Introduction

This gene encodes the protein shares similarity with BRE1 of S. cerevisiae. This human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. Including many genes that are induced by an epidermal growth factor, this protein also suppresses the expression of several protooncogenes and growth-related genes. In contrast, this protein positively regulates the p53 tumor suppressor and acts as a putative tumor suppressor as well as numerous histone H2A and H2B genes. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).

Entrez Gene ID

56254

UniProt ID

Q5VTR2

Alternative Names

BRE1; BRE1A; hBRE1

Function

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Biological Process

Biological Process chromatin organizationIEA:UniProtKB-KW
Biological Process histone H2B conserved C-terminal lysine ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process histone H2B ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process histone monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of histone methylationIEA:Ensembl
Biological Process positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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