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Mouse Anti-RNF20 Monoclonal Antibody (1D7) (CBMAB-0055-LY)

This product is mouse monoclonal antibody recognizes RNF20 of human. The antibody 1D7 immunoassay techniques such as: MA, WB.
See all RNF20 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D7
Antibody Isotype
IgG2b
Application
MA, WB

Basic Information

Immunogen
Recombinant peptide (Protein Domain)
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
RNF20
Introduction
This gene encodes the protein shares similarity with BRE1 of S. cerevisiae. This human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. Including many genes that are induced by an epidermal growth factor, this protein also suppresses the expression of several protooncogenes and growth-related genes. In contrast, this protein positively regulates the p53 tumor suppressor and acts as a putative tumor suppressor as well as numerous histone H2A and H2B genes. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).
Entrez Gene ID
56254
UniProt ID
Q5VTR2
Alternative Names
BRE1; BRE1A; hBRE1
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Biological Process
Biological Process chromatin organizationIEA:UniProtKB-KW
Biological Process histone H2B conserved C-terminal lysine ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process histone H2B ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process histone monoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of histone methylationIEA:Ensembl
Biological Process positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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