Mouse Anti-ROCK2 Recombinant Antibody (1-00E-012) (CBMAB-R3126-CN)

Rho Associated Coiled-Coil Containing Protein Kinase 2

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Rho Associated Coiled-Coil Containing Protein Kinase 2; Rho-Associated, Coiled-Coil-Containing Protein Kinase II; P164 ROCK-2; EC 2.7.11.1; ROCK-II; Rho-Associated, Coiled-Coil-Containing Protein Kinase 2;

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Biological Process actin cytoskeleton organizationISS:BHF-UCL
Biological Process actomyosin structure organizationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process aortic valve morphogenesisISS:BHF-UCL
Biological Process blood vessel diameter maintenanceISS:ARUK-UCL
Biological Process cellular response to acetylcholineISS:ARUK-UCL
Biological Process cellular response to testosterone stimulusManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process centrosome duplicationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process cortical actin cytoskeleton organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process embryonic morphogenesisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process epithelial to mesenchymal transitionISS:BHF-UCL
Biological Process I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic cytokinesisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process modulation by host of viral processManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Biological Process mRNA destabilizationISS:ARUK-UCL
Biological Process negative regulation of angiogenesisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of bicellular tight junction assemblyManual Assertion Based On ExperimentIGI:UniProtKB
Biological Process negative regulation of biomineral tissue developmentISS:BHF-UCL
Biological Process negative regulation of gene expressionISS:ARUK-UCL
Biological Process negative regulation of myosin-light-chain-phosphatase activityManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of nitric oxide biosynthetic processManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process negative regulation of protein localization to lysosomeManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process peptidyl-serine phosphorylationManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process peptidyl-threonine phosphorylationManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process positive regulation of amyloid precursor protein catabolic processManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process positive regulation of amyloid-beta formationManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic processManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process positive regulation of cardiac muscle hypertrophyISS:ARUK-UCL
Biological Process positive regulation of cell migrationManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process positive regulation of centrosome duplicationIEA:InterPro
Biological Process positive regulation of connective tissue growth factor productionISS:ARUK-UCL
Biological Process positive regulation of connective tissue replacementISS:ARUK-UCL
Biological Process positive regulation of endothelial cell migrationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of fibroblast growth factor productionISS:ARUK-UCL
Biological Process positive regulation of gene expressionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of MAPK cascadeISS:ARUK-UCL
Biological Process positive regulation of protein localization to early endosomeManual Assertion Based On ExperimentIMP:ARUK-UCL
Biological Process positive regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of stress fiber assemblyManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process protein localization to plasma membraneManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of actin cytoskeleton organizationManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process regulation of angiotensin-activated signaling pathwayISS:ARUK-UCL
Biological Process regulation of cell adhesionManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process regulation of cell junction assemblyManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of cell motilityManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process regulation of cellular response to hypoxiaISS:ARUK-UCL
Biological Process regulation of circadian rhythmISS:UniProtKB
Biological Process regulation of establishment of cell polarityManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process regulation of establishment of endothelial barrierManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of focal adhesion assemblyManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process regulation of keratinocyte differentiationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of nervous system processISS:ARUK-UCL
Biological Process regulation of protein metabolic processBy SimilarityISS:ARUK-UCL
Biological Process regulation of stress fiber assemblyManual Assertion Based On ExperimentTAS:UniProtKB
Biological Process response to angiotensinISS:ARUK-UCL
Biological Process response to ischemiaISS:ARUK-UCL
Biological Process response to transforming growth factor betaISS:ARUK-UCL
Biological Process Rho protein signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process rhythmic processIEA:UniProtKB-KW
Biological Process smooth muscle contractionManual Assertion Based On ExperimentTAS:UniProtKB

Cytoplasm
Cell membrane
Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasmic, and associated with actin microfilaments and the plasma membrane.

Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.