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Mouse Anti-RSF1 Recombinant Antibody (3E6) (CBMAB-A3759-LY)

The product is antibody recognizes HBXAP. The antibody 3E6 immunoassay techniques such as: WB, ELISA.
See all RSF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3E6
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
HBXAP (NP_057662.3, 1343 a.a. ~ 1441 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Remodeling And Spacing Factor 1
Introduction
HBXAP is involved in transcription repression, transcription coactivation when associated with hepatitis B virus X protein (HBX), and chromatin remodeling and spacing when associated with SNF2H (MIM 603375).[supplied by OMIM
Entrez Gene ID
51773
UniProt ID
Q96T23
Alternative Names
HBXAP; RSF-1; XAP8; p325
Function
Regulatory subunit of the ATP-dependent RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:12972596, PubMed:28801535).
Binds to core histones together with SMARCA5, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed:12972596).
Directly stimulates the ATPase activity of SMARCA1 and SMARCA5 in the RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, respectively (PubMed:28801535).
The RSF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the RSF-5 ISWI chromatin-remodeling complex (PubMed:28801535).
The complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535).
Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain (PubMed:11944984, PubMed:11788598).
Biological Process
Biological Process chromatin assemblyManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process chromatin remodelingManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process DNA-templated transcription initiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of DNA bindingManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process nucleosome assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process positive regulation of viral transcriptionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:ComplexPortal
Cellular Location
Nucleus
Localization is diffuse during mitosis (PubMed:12972596).
Co-localizes with SMARCA5 in the nucleus (PubMed:12972596).
PTM
Phosphorylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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