Mouse Anti-S. pombe Cig2 Recombinant Antibody (CIG2 3A11/5) (CBMAB-V208-0083-CQ)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
CIG2 3A11/5
Application
WB, IHC, IF
Immunogen
S. pombe Cig2 purified protein (bacterial expression)
Specificity
S. pombe
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
G1/S-specific B-type cyclin Cig2
Introduction
Essential for the control of the cell cycle at the G2/M and G1/S (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. Interaction with res2 promotes the phosphorylation of res1 and inhibits MBF-dependent gene transcription. Forms an autoregulating feedback-inhibition loop with MBF which is important for normal regulation of the cell cycle. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Negatively regulates conjugation via interacting with cell cycle 'start' genes. Degraded by skp1, pop1 and pop2 in the G2 and M phases of the cell cycle.
Entrez Gene ID
2543481
UniProt ID
P36630
Alternative Names
cyc17; cig2; G1/S-specific B-type cyclin Cig2
Function
Essential for the control of the cell cycle at the G2/M and G1/S (mitosis) transition. Interacts with the cdc2 protein kinase to form MPF. Interaction with res2 promotes the phosphorylation of res1 and inhibits MBF-dependent gene transcription. Forms an autoregulating feedback-inhibition loop with MBF which is important for normal regulation of the cell cycle. G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis. Negatively regulates conjugation via interacting with cell cycle 'start' genes. Degraded by skp1, pop1 and pop2 in the G2 and M phases of the cell cycle.
Biological Process
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process DNA recombinationManual Assertion Based On ExperimentIMP:PomBase
Biological Process G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:PomBase
Biological Process G2/M transition of mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mitotic cell cycle phase transitionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mitotic G1 cell size control checkpoint signalingManual Assertion Based On ExperimentIGI:PomBase
Biological Process negative regulation of conjugation with cellular fusionManual Assertion Based On ExperimentIMP:PomBase
Biological Process positive regulation of G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentIMP:PomBase
Biological Process regulation of cell cycle switching, mitotic to meiotic cell cycleManual Assertion Based On ExperimentIMP:PomBase
Biological Process regulation of cyclin-dependent protein serine/threonine kinase activityManual Assertion Based On ExperimentIBA:GO_Central
Biological Process traversing start control point of mitotic cell cycleManual Assertion Based On ExperimentIGI:PomBase
Cellular Location
Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body
PTM
Phosphorylated.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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