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Mouse Anti-SELPLG Recombinant Antibody (CBYC-P826) (CBMAB-P3638-YC)

Provided herein is a Mouse monoclonal antibody against Human Selectin P Ligand. The antibody can be used for immunoassay techniques, such as FC.
See all SELPLG antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-P826
Antibody Isotype
IgG2a
Application
FC

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Selectin P Ligand
Introduction
SELPLG is a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E-and L-selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.
Entrez Gene ID
6404
UniProt ID
Q14242
Alternative Names
PSGL-1; CD162; CLA; PSGL1
Function
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
(Microbial infection) Acts as a receptor for enterovirus 71.
Biological Process
Biological Process cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process cellular response to interleukin-6Manual Assertion Based On ExperimentIDA:MGI
Biological Process hemopoiesisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process leukocyte adhesive activationISS:UniProtKB
Biological Process leukocyte migrationManual Assertion Based On ExperimentIDA:CAFA
Biological Process leukocyte tethering or rollingManual Assertion Based On ExperimentIDA:CAFA
Cellular Location
Membrane
Topology
Extracellular: 18-320
Helical: 321-341
Cytoplasmic: 342-412
PTM
Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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