Mouse Anti-SF3B2 Recombinant Antibody (CBXS-2085) (CBMAB-S4851-CQ)

Name: Mouse Anti-SF3B2 Recombinant Antibody (CBXS-2085)
SKU: CBMAB-S4851-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBXS-2085

Application

WB, IP, IF, ELISA

Specificity

Mouse, Rat, Human

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

splicing factor 3b, subunit 2, 145kDa

Introduction

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Entrez Gene ID

Human	10992
Mouse	319322
Rat	293671

UniProt ID

Human	Q13435
Mouse	Q921M3
Rat	D3ZMS1

Alternative Names

Splicing Factor 3b Subunit 2; Splicing Factor 3b, Subunit 2, 145kDa; Splicing Factor 3b, Subunit 2, 145kD; SAP 145; SF3B145; SAP145; Pre-MRNA Splicing Factor SF3b 145 KDa Subunit;

Function

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643).
SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).
May also be involved in the assembly of the 'E' complex (PubMed:10882114).
Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).

Biological Process

Biological Process mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Biological Process mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process RNA splicingManual Assertion Based On ExperimentTAS:ProtInc
Biological Process U2-type prespliceosome assembly1 PublicationIC:ComplexPortal

Cellular Location

Nucleus
Nucleus speckle

PTM

Methylation at Arg-508 by PRMT9 is required for the interaction with SMN1.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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