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Mouse Anti-SGO1 Recombinant Antibody (CBXS-3031) (CBMAB-S5777-CQ)

This product is a mouse antibody that recognizes SGO1. The antibody CBXS-3031 can be used for immunoassay techniques such as: ELISA, IP, WB.
See all SGO1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-3031
Antibody Isotype
IgG2a, κ
Application
ELISA, IP, WB

Basic Information

Immunogen
Full length recombinant protein corresponding to aa1-293 from human SGOL1 (AAH17867) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Shugoshin 1
Introduction
The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
151648
UniProt ID
Q5FBB7
Alternative Names
CAID; NY-BR-85; SGO; SGOL1
Function
Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase.
Biological Process
Biological Process attachment of spindle microtubules to kinetochoreManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process centriole-centriole cohesionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process chromosome segregationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process homologous chromosome segregationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process meiotic chromosome segregationManual Assertion Based On ExperimentIMP:MGI
Biological Process meiotic sister chromatid cohesionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process mitotic sister chromatid cohesion, centromericManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process mitotic sister chromatid segregationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Chromosome, centromere
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, spindle pole
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase (PubMed:16541025).
Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A (PubMed:16580887)(PubMed:16541025)(PubMed:15604152).
Colocalizes with NEK2 at the kinetochore (PubMed:17621308).
Colocalizes with and SS18L1 at the kinetochore (PubMed:16582621).
Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore (PubMed:17617734).
Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis (PubMed:16582621).
Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent (PubMed:16582621).
Isoform 3 does not localize to kinetochores during any stages of the cell cycle (PubMed:16582621).
Involvement in disease
Chronic atrial and intestinal dysrhythmia (CAID):
A disease characterized by dysregulation of the cardiac sinus node resulting in sick sinus syndrome, in association with chronic intestinal pseudo-obstruction, a disorder of gastrointestinal motility in which intestinal obstruction occurs in the absence of a mechanical obstacle.
PTM
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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