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Mouse Anti-SH3RF1 Recombinant Antibody (3H3) (CBMAB-A8102-LY)

The product is antibody recognizes SH3MD2. The antibody 3H3 immunoassay techniques such as: WB, ELISA.
See all SH3RF1 antibodies
Published Data
Fig.1 Genetic screens identify SH3RF1 as a specific mediator of oncogenic PI3K/AKT-induced differentiation but not proliferation.
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Fig.2 Coimmunoprecipitation assay shows reciprocal binding between endogenous FAT1 and SH3RF1 in MDA-MB-231 cell lysates.
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Fig.3 Western blotting for FAT1 using antibodies directed against the C-terminal domain after transient siRNA-mediated knockdown of SH3RF1 in MDA-MB-231 cells.
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Fig.4 Transient siRNA knockdown of SH3RF1 lead to increased FAT1 protein in both HEK293 and MDA-MB-231 cells.
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Fig.5 Sh3rf2 and POSH physically interact in mammalian cells.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3H3
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
SH3MD2 (NP_065921, 790 a.a. ~ 888 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
SH3 Domain Containing Ring Finger 1
Introduction
This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. [provided by RefSeq]
Entrez Gene ID
57630
UniProt ID
Q7Z6J0
Alternative Names
FLJ21602; KIAA1494; POSH; RNF142; SH3MD2
Function
Has E3 ubiquitin-protein ligase activity. In the absence of an external substrate, it can catalyze self-ubiquitination (PubMed:15659549, PubMed:20696164).
Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis (PubMed:19710010).
Acts as a scaffold protein that coordinates with MAPK8IP1/JIP1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the differentiation of CD4+ and CD8+ T-cells and promotes T-helper 1 (Th1) cell differentiation. Regulates the activation of MAPK8/JNK1 and MAPK9/JNK2 in CD4+ T-cells and the activation of MAPK8/JNK1 in CD8+ T-cells. Plays a crucial role in the migration of neocortical neurons in the developing brain. Controls proper cortical neuronal migration and the formation of proximal cytoplasmic dilation in the leading process (PCDLP) in migratory neocortical neurons by regulating the proper localization of activated RAC1 and F-actin assembly (By similarity).
(Microbial infection) Plays an essential role in the targeting of HIV-1 Gag to the plasma membrane, this function is dependent on it's RING domain, and hence it's E3 ligase activity.
Biological Process
Biological Process negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of cysteine-type endopeptidase activity involved in apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of extrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process neuron migrationISS:UniProtKB
Biological Process positive regulation of JNK cascadeISS:UniProtKB
Biological Process positive regulation of proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein autoubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of CD4-positive, alpha-beta T cell differentiationISS:UniProtKB
Biological Process regulation of CD8-positive, alpha-beta T cell proliferationISS:UniProtKB
Cellular Location
Cytoplasm, perinuclear region
Cell projection, lamellipodium
Golgi apparatus, trans-Golgi network
Colocalizes, with AKT2, in lamellipodia (By similarity).
Colocalizes, with HERP1, in trans-Golgi network
PTM
Phosphorylated at Ser-304 by AKT1 and AKT2. When phosphorylated, it has reduced ability to bind Rac.
Autoubiquitinated (PubMed:20696164).
Ubiquitinated by SH3RF2, leading to proteasome-mediated degradation (By similarity).
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For research use only. Not intended for any clinical use.

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