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Mouse Anti-SHMT2 Recombinant Antibody (CBXS-5772) (CBMAB-S0631-CQ)

This product is a mouse antibody that recognizes SHMT2. The antibody CBXS-5772 can be used for immunoassay techniques such as: WB.
See all SHMT2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-5772
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
Recombinant full length human SHMT2(NP_005403)
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
serine hydroxymethyltransferase 2 (mitochondrial)
Introduction
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
6472
UniProt ID
P34897
Alternative Names
Serine Hydroxymethyltransferase 2; Serine Hydroxymethyltransferase 2 (Mitochondrial); Glycine Hydroxymethyltransferase; Serine Methylase; EC 2.1.2.1; SHMT; Epididymis Secretory Sperm Binding Protein Li 51e; Glycine Auxotroph A, Human Complement For Hamster;
Function
Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:29364879, PubMed:33015733 PubMed:25619277, PubMed:33015733).
Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277).
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188).
Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29452640, PubMed:29364879).
Associates with mitochondrial DNA (PubMed:18063578).
In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985).
Biological Process
Biological Process folic acid metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process glycine biosynthetic process from serineManual Assertion Based On ExperimentIBA:GO_Central
Biological Process glycine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process L-serine biosynthetic processIEA:Ensembl
Biological Process L-serine catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process L-serine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process one-carbon metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of cell population proliferationIEA:Ensembl
Biological Process protein homotetramerizationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein K63-linked deubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein tetramerizationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process purine nucleobase biosynthetic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of aerobic respirationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of mitochondrial translationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of oxidative phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process response to type I interferonManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process tetrahydrofolate interconversionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process tetrahydrofolate metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Mitochondrion
Mitochondrion matrix, mitochondrion nucleoid
Mitochondrion inner membrane
Cytoplasm
Nucleus
Mainly localizes in the mitochondrion. Also found in the cytoplasm and nucleus as part of the BRISC complex (PubMed:24075985).
Involvement in disease
Neurodevelopmental disorder with cardiomyopathy, spasticity, and brain abnormalities (NEDCASB):
An autosomal recessive neurodevelopmental disorder characterized by global developmental delay, moderate to severe intellectual disability, spastic paraparesis, ataxia, and/or peripheral neuropathy. Patients also exhibit dysmorphic features and congenital microcephaly. Most affected individuals develop progressive hypertrophic cardiomyopathy in childhood or have cardiac developmental anomalies. Brain imaging shows corpus callosum abnormalities in all patients, and perisylvian polymicrogyria-like pattern in some individuals.
PTM
Succinylation at Lys-280 inhibits the hydroxymethyltransferase activity. Desuccinylation by SIRT5 restores the activity, leading to promote cell proliferation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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