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Mouse Anti-SIRT4 Recombinant Antibody (CBXS-5790) (CBMAB-S0648-CQ)

This product is a mouse antibody that recognizes SIRT4. The antibody CBXS-5790 can be used for immunoassay techniques such as: ELISA, WB, IF, FC.
See all SIRT4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-5790
Antibody Isotype
IgG1
Application
ELISA, WB, IF, FC

Basic Information

Immunogen
Recombinant fragment of human SIRT4
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Introduction
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Entrez Gene ID
23409
UniProt ID
Q9Y6E7
Alternative Names
Sirtuin 4; NAD-Dependent ADP-Ribosyltransferase Sirtuin-4; NAD-Dependent Protein Deacetylase Sirtuin-4; Regulatory Protein SIR2 Homolog 4; SIR2-Like Protein 4; EC 3.5.1.-; SIR2L4;
Function
Acts as NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase (PubMed:16959573, PubMed:17715127, PubMed:24052263, PubMed:25525879).
Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications (PubMed:24052263, PubMed:25525879).
Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879).
Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity (PubMed:16959573, PubMed:17715127).
Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127).
In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation (PubMed:23663782).
Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782).
Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity).
Does not seem to deacetylate PC (PubMed:23438705).
Controls fatty acid oxidation by inhibiting PPARA transcriptional activation (PubMed:24043310).
Impairs SIRT1-PPARA interaction probably through the regulation of NAD+ levels (PubMed:24043310).
Down-regulates insulin secretion (PubMed:17715127).
Biological Process
Biological Process cellular response to DNA damage stimulusISS:UniProtKB
Biological Process cellular response to hypoxiaIEA:Ensembl
Biological Process glutamine metabolic processISS:UniProtKB
Biological Process mitochondrion organizationTAS:Reactome
Biological Process negative regulation of cardiac muscle cell apoptotic processIEA:Ensembl
Biological Process negative regulation of fatty acid oxidationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of insulin secretionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of protein processing involved in protein targeting to mitochondrionIEA:Ensembl
Biological Process peptidyl-lysine deacetylationISS:UniProtKB
Biological Process positive regulation of lipid biosynthetic processISS:UniProtKB
Biological Process protein ADP-ribosylationISS:UniProtKB
Biological Process protein deacetylationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of glutamine family amino acid metabolic processIEA:Ensembl
Biological Process regulation of pyruvate dehydrogenase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process tricarboxylic acid metabolic processISS:UniProtKB
Cellular Location
Mitochondrion matrix
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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