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Rabbit Anti-SLC25A5 Recombinant Antibody (E2B9D) (CBMAB-CP2465-LY)

The product is antibody recognizes SLC25A5. The antibody E2B9D immunoassay techniques such as: WB,IP.
See all SLC25A5 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
E2B9D
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly156 of human ANT2/SLC25A5 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Solute Carrier Family 25 Member 51
Entrez Gene ID
Human292
Mouse11740
Rat25176
Monkey693502
UniProt ID
HumanP05141
MouseP51881
RatQ09073
MonkeyI0FR46
Alternative Names
Solute Carrier Family 25 Member 51; Mitochondrial Carrier Triple Repeat Protein 1; Mitochondrial Carrier Triple Repeat 1; MCART1; Solute Carrier Family 25; Member 51; CG7943;
Function
ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity).
Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity).
In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (By similarity).
Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity).
Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A5/ANT2 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity).
Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity).
Probably mediates mitochondrial uncoupling in tissues that do not express UCP1 (By similarity).
Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (PubMed:31883789).
It is however unclear if SLC25A5/ANT2 constitutes a pore-forming component of mPTP or regulates it (By similarity).
Acts as a regulator of mitophagy independently of ADP:ATP antiporter activity: promotes mitophagy via interaction with TIMM44, leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (By similarity).
As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed:20797633).
Biological Process
Biological Process adaptive thermogenesisISS:UniProtKB
Biological Process adenine nucleotide transportManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process B cell differentiationISS:UniProtKB
Biological Process chromosome segregationIEA:UniProtKB-KW
Biological Process erythrocyte differentiationISS:UniProtKB
Biological Process mitochondrial ADP transmembrane transportISS:UniProtKB
Biological Process mitochondrial ATP transmembrane transportISS:UniProtKB
Biological Process negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of mitophagyISS:UniProtKB
Biological Process regulation of mitochondrial membrane permeabilityISS:UniProtKB
Cellular Location
Mitochondrion inner membrane
Membrane
May localize to non-mitochondrial membranes.
Topology
Mitochondrial intermembrane: 1-7
Helical: 8-37
Mitochondrial matrix: 38-74
Helical: 75-99
Mitochondrial intermembrane: 100-109
Helical: 110-130
Mitochondrial matrix: 131-178
Helical: 179-199
Mitochondrial intermembrane: 200-210
Helical: 211-231
Mitochondrial matrix: 232-273
Helical: 274-291
Mitochondrial intermembrane: 292-298
PTM
Trimethylated by ANTKMT at Lys-52.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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