Mouse Anti-SPAG16 Recombinant Antibody (CBXS-2733) (CBMAB-S5484-CQ)

Name: Mouse Anti-SPAG16 Recombinant Antibody (CBXS-2733)
SKU: CBMAB-S5484-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBXS-2733

Application

ELISA, WB

Immunogen

SPAG16 (NP_001020607.1, aa1-183) Full length recombinant protein with GST tag

Specificity

Human, Rat

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

sperm associated antigen 16

Introduction

Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).

Entrez Gene ID

Human	79582
Rat	501158

UniProt ID

Human	Q8N0X2
Rat	B0BMX7

Alternative Names

Sperm Associated Antigen 16; Pf20 Protein Homolog; PF20; Sperm-Associated Antigen 16 Protein; Sperm-Associated WD Repeat Protein; WD Repeat Domain 29; WDR29;

Function

Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).

Biological Process

Biological Process axoneme assemblyManual Assertion Based On ExperimentIMP:GO_Central
Biological Process cerebrospinal fluid circulationIEA:Ensembl
Biological Process cilium assemblyISS:UniProtKB
Biological Process mucociliary clearanceIEA:Ensembl
Biological Process sperm axoneme assemblyIEA:Ensembl

Cellular Location

Cytoplasm
Cytoplasm, cytoskeleton, flagellum axoneme
Cytoplasm, cytoskeleton, cilium axoneme
Cell projection, cilium, flagellum
Detected on the sperm flagellum axoneme. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules (By similarity).

PTM

Phosphorylated by TSSK2.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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