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Mouse Anti-SPAG16 Recombinant Antibody (CBXS-2733) (CBMAB-S5484-CQ)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBXS-2733
Application
ELISA, WB

Basic Information

Immunogen
SPAG16 (NP_001020607.1, aa1-183) Full length recombinant protein with GST tag
Specificity
Human, Rat
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
sperm associated antigen 16
Introduction
Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).
Entrez Gene ID
Human79582
Rat501158
UniProt ID
HumanQ8N0X2
RatB0BMX7
Alternative Names
Sperm Associated Antigen 16; Pf20 Protein Homolog; PF20; Sperm-Associated Antigen 16 Protein; Sperm-Associated WD Repeat Protein; WD Repeat Domain 29; WDR29;
Function
Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).
Biological Process
Biological Process axoneme assemblyManual Assertion Based On ExperimentIMP:GO_Central
Biological Process cerebrospinal fluid circulationIEA:Ensembl
Biological Process cilium assemblyISS:UniProtKB
Biological Process mucociliary clearanceIEA:Ensembl
Biological Process sperm axoneme assemblyIEA:Ensembl
Cellular Location
Cytoplasm
Cytoplasm, cytoskeleton, flagellum axoneme
Cytoplasm, cytoskeleton, cilium axoneme
Cell projection, cilium, flagellum
Detected on the sperm flagellum axoneme. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules (By similarity).
PTM
Phosphorylated by TSSK2.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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