Mouse Anti-SPAG8 Recombinant Antibody (CBXS-2911) (CBMAB-S5660-CQ)

Name: Mouse Anti-SPAG8 Recombinant Antibody (CBXS-2911)
SKU: CBMAB-S5660-CQ
Rating: 5 (1 reviews)

See all SPAG8 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXS-2911

Application

ELISA, WB

Immunogen

Full length recombinant corresponding to aa1-502 from human SPAG8 (AAH19247) with GST tag

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

sperm associated antigen 8

Introduction

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Entrez Gene ID

26206

UniProt ID

Q99932

Alternative Names

Sperm Associated Antigen 8; Sperm Membrane Protein BS-84; Sperm Membrane Protein 1; HSD-1; Testicular Tissue Protein Li 177; Sperm-Associated Antigen 8; CILD28;

Function

Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau (By similarity).
Involved in the acrosome reaction and in binding of sperm to the zona pellucida (By similarity).
Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis (PubMed:19548270).
May play a role in fertility and microtubule formation through interaction with RANBP9 (PubMed:10500252).

Biological Process

Biological Process cell cycleIEA:UniProtKB-KW
Biological Process cell differentiationIEA:UniProtKB-KW
Biological Process positive regulation of protein bindingIEA:Ensembl
Biological Process positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process single fertilizationIEA:UniProtKB-KW
Biological Process spermatogenesisIEA:UniProtKB-KW

Cellular Location

Cytoplasm
Nucleus
Cytoplasmic vesicle, secretory vesicle, acrosome
Cytoplasm, cytoskeleton, microtubule organizing center
Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, cilium axoneme
In mature sperm cells, detected in the acrosomal region of the head and in the middle piece of the tail (By similarity).
Localized to the nucleus and cytoplasm of spermatocytes and round spermatids while, in elongating spermatids, expressed in the cytoplasm but not in the nucleus (By similarity).
During the cell cycle, localized on the microtubule-organizing center (MTOC) during prophase. In metaphase, extends along spindle microtubules. In anaphase, detected on the astral microtubules and mid-zone. In telophase, remains at the mid-zone. After cytokinesis, returns to the MTOC (PubMed:19548270).
Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme (By similarity).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-SPAG8 Recombinant Antibody (CBXS-2911)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry