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Mouse Anti-SPRED2 Recombinant Antibody (6G8) (CBMAB-S2887-CQ)

This product is a mouse antibody that recognizes SPRED2. The antibody 6G8 can be used for immunoassay techniques such as: WB, IP, ELISA.
See all SPRED2 antibodies
Published Data
Fig.1 Western blot confirming expression of the SPRED2-Myc construct in PC3M cells.
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Fig.2 EGF stimulates Spred-2 phosphorylation and ubiquitination.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
6G8
Application
WB, IP, ELISA

Basic Information

Specificity
Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
sprouty-related, EVH1 domain containing 2
Introduction
SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).
Entrez Gene ID
200734
UniProt ID
Q7Z698
Alternative Names
Sprouty Related EVH1 Domain Containing 2; Spred-2; Sprouty Protein With EVH-1 Domain 2, Related Sequence; Sprouty-Related, EVH1 Domain-Containing Protein 2;
Function
Negatively regulates Ras signaling pathways and downstream activation of MAP kinases (PubMed:15683364).
Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity).
Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity).
Biological Process
Biological Process negative regulation of epithelial to mesenchymal transitionISS:UniProtKB
Biological Process negative regulation of ERK1 and ERK2 cascadeISS:UniProtKB
Biological Process negative regulation of lens fiber cell differentiationISS:UniProtKB
Biological Process negative regulation of MAPK cascadeISS:UniProtKB
Biological Process negative regulation of peptidyl-threonine phosphorylationManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process negative regulation of transforming growth factor beta receptor signaling pathwayISS:UniProtKB
Biological Process positive regulation of DNA damage response, signal transduction by p53 class mediatorBy SimilarityISS:BHF-UCL
Biological Process regulation of protein deacetylationBy SimilarityISS:BHF-UCL
Cellular Location
Cell membrane
Cytoplasmic vesicle, secretory vesicle membrane
Cytoplasm
Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules.
PTM
Phosphorylated on serine and threonine residues (PubMed:15683364).
Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination (PubMed:17094949).
Ubiquitinated; leading to degradation by the proteasome.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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