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Mouse Anti-SPTLC1 Recombinant Antibody (CBYJL-2342) (CBMAB-L2645-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Serine Palmitoyltransferase Long Chain Base Subunit 1 (SPTLC1). The antibody can be used for immunoassay techniques, such as WB, IF.
See all SPTLC1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Rat, Mouse
Clone
CBYJL-2342
Antibody Isotype
IgG1
Application
WB, IF

Basic Information

Immunogen
Mouse LCB1 aa 121-238.
Specificity
Human, Rat, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 121-238

Target

Full Name
serine palmitoyltransferase, long chain base subunit 1
Introduction
SPTLC1 is a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13.
Entrez Gene ID
Human10558
Mouse268656
Rat361213
UniProt ID
HumanO15269
MouseO35704
RatD4A2H2
Alternative Names
Serine Palmitoyltransferase Long Chain Base Subunit 1; Long Chain Base Biosynthesis Protein 1; Serine-Palmitoyl-CoA Transferase 1; EC 2.3.1.50; LCB 1; SPT 1; LCB1; SPT1
Function
Serine palmitoyltransferase (SPT) (PubMed:19416851).
The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core (PubMed:19416851).
The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed:19416851).
The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed:19416851).
The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed:19416851).
Required for adipocyte cell viability and metabolic homeostasis (By similarity).
Biological Process
Biological Process ceramide biosynthetic processManual Assertion Based On ExperimentIDA:MGI
Biological Process positive regulation of lipophagyManual Assertion Based On ExperimentIDA:MGI
Biological Process regulation of fat cell apoptotic processISS:UniProtKB
Biological Process sphinganine biosynthetic processIEA:Ensembl
Biological Process sphingolipid biosynthetic processManual Assertion Based On ExperimentIDA:MGI
Biological Process sphingolipid metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process sphingomyelin biosynthetic processIEA:Ensembl
Biological Process sphingosine biosynthetic processManual Assertion Based On ExperimentIDA:ComplexPortal
Cellular Location
Endoplasmic reticulum membrane
Involvement in disease
Neuropathy, hereditary sensory and autonomic, 1A (HSAN1A):
A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1A is an autosomal dominant axonal form with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations.
Topology
Lumenal: 1-15
Helical: 16-36
Cytoplasmic: 37-473
PTM
Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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