Rabbit Anti-SRR Recombinant Antibody (
12B2) (V2LY-0725-LY1513)

Name: Rabbit Anti-SRR Recombinant Antibody (12B2)
SKU: V2LY-0725-LY1513
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-SRR Recombinant Antibody (12B2) (V2LY-0725-LY1513) in WB

Western Blot
Positive WB detected in: A549 whole cell lysate, U87 whole cell lysate
All lanes: SRR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa

Rabbit Anti-SRR Recombinant Antibody (12B2) (V2LY-0725-LY1513) in FC

Overlay Peak curve showing HepG2 cells stained with V2LY-0725-LY1513 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

12B2

Application

ELISA, WB, FC

Immunogen

A synthesized peptide derived from human SRR.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Serine Racemase

Entrez Gene ID

63826

UniProt ID

Q9GZT4

Function

Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.

Biological Process

Biological Process agingIEA:Ensembl
Biological Process brain developmentIEA:Ensembl
Biological Process D-serine biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process D-serine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process L-serine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process pyruvate biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process response to lipopolysaccharideManual Assertion Based On ExperimentIEP:UniProtKB
Biological Process response to morphineIEA:Ensembl
Biological Process response to xenobiotic stimulusIEA:Ensembl
Biological Process serine family amino acid metabolic processISS:UniProtKB

Cellular Location

apical part of cell
cytoplasm
cytosol
neuronal cell body
plasma membrane

PTM

S-nitrosylated, leading to decrease the enzyme activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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