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Recombinant Mouse Anti-SRSF2 Recombinant Antibody (1SC-4F11) (CBMAB-XB1066-YC)

Provided herein is a Mouse Recombinant Antibody against Serine And Arginine Rich Splicing Factor 2. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all SRSF2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
1SC-4F11
Antibody Isotype
IgG1, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
Synthetic peptide coupled to Ovalbumin (C)-KSPPKSPEEEGAVSS corresponding to aa 207-221 of human SC35
Specificity
Human, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Serine And Arginine Rich Splicing Factor 2
Introduction
SRSF2 is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation.
Entrez Gene ID
Human6427
Mouse20382
UniProt ID
HumanQ01130
MouseQ62093
Alternative Names
Serine And Arginine Rich Splicing Factor 2; Splicing Factor, Arginine/Serine-Rich 2; Serine/Arginine-Rich Splicing Factor 2; Splicing Component, 35 KDa; SR Splicing Factor 2; Splicing Factor SC35; SFRS2;
Function
Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
Biological Process
Biological Process mRNA processingManual Assertion Based On ExperimentTAS:ProtInc
Biological Process mRNA splicing, via spliceosomeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of alternative mRNA splicing, via spliceosomeIEA:InterPro
Biological Process RNA splicingManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus
Nucleus, nucleoplasm
Nucleus speckle
Phosphorylation by SRPK2 provokes its redistribution from the nuclear speckle to nucleoplasm.
PTM
Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by SRPK2 and this causes its redistribution from the nuclear speckle to nucleoplasm and controls cell fate decision in response to cisplatin treatment. KAT5/TIP60 inhibits its phosphorylation by preventing SRPK2 nuclear translocation.
Acetylation on Lys-52 by KAT5/TIP60 promotes its proteasomal degradation. This effect is counterbalanced by HDAC6, which positively controls SRSF2 protein level by deacetylating it and preventing its proteasomal degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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