Mouse Anti-STK11 Recombinant Antibody (CBYJL-2349) (CBMAB-L2654-YJ)

STK11

STK11 is a member of the serine/threonine kinase family and regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been found but have not been thoroughly characterized.

PJS; LKB1; hLKB1

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316).
Isoform 2
Has a role in spermiogenesis.

Activation of protein kinase activityManual Assertion Based On ExperimentIDA:MGI
AnoikisManual Assertion Based On ExperimentIMP:BHF-UCL
AutophagyIEA:UniProtKB-KW
AxonogenesisIEA:Ensembl
Canonical Wnt signaling pathwayIEA:Ensembl
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to UV-BManual Assertion Based On ExperimentIDA:UniProtKB
Dendrite extensionIEA:Ensembl
Establishment of cell polarityManual Assertion Based On ExperimentIMP:UniProtKB
G1 to G0 transitionManual Assertion Based On ExperimentIDA:UniProtKB
Glucose homeostasisISS:UniProtKB
Golgi localizationIEA:Ensembl
Intrinsic apoptotic signaling pathway by p53 class mediatorManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of cell growthISS:UniProtKB
Negative regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Negative regulation of epithelial cell proliferation involved in prostate gland developmentIEA:Ensembl
Negative regulation of TORC1 signalingManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Peptidyl-threonine phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of autophagyManual Assertion Based On ExperimentIMP:ParkinsonsUK-UCL
Positive regulation of axonogenesisIEA:Ensembl
Positive regulation of gluconeogenesisIEA:Ensembl
Positive regulation of protein localization to nucleusIEA:Ensembl
Positive regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of vesicle transport along microtubuleManual Assertion Based On ExperimentIMP:UniProtKB
Positive thymic T cell selectionIEA:Ensembl
Protein autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein dephosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell cycleTAS:Reactome
Regulation of cell growthISS:UniProtKB
Regulation of dendrite morphogenesisIEA:Ensembl
Regulation of protein kinase B signalingIEA:Ensembl
Regulation of signal transduction by p53 class mediatorTAS:Reactome
Response to activityIEA:Ensembl
Response to glucagonIEA:Ensembl
Response to ionizing radiationISS:UniProtKB
Response to lipidIEA:Ensembl
Response to thyroid hormoneIEA:Ensembl
SpermatogenesisIEA:UniProtKB-KW
T cell receptor signaling pathwayIEA:Ensembl
Tissue homeostasisIEA:Ensembl
Vasculature developmentISS:UniProtKB

Nucleus
Cytoplasm
Membrane
Mitochondrion
A small fraction localizes at membranes (By similarity).
Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to the mitochondrion during apoptosis. PTEN promotes cytoplasmic localization.
Isoform 2
Nucleus
Cytoplasm
Predominantly nuclear, but translocates to the cytoplasm in response to metformin or peroxynitrite treatment.

Peutz-Jeghers syndrome (PJS):
An autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Testicular germ cell tumor (TGCT):
A common malignancy in males representing 95% of all testicular neoplasms. TGCTs have various pathologic subtypes including: unclassified intratubular germ cell neoplasia, seminoma (including cases with syncytiotrophoblastic cells), spermatocytic seminoma, embryonal carcinoma, yolk sac tumor, choriocarcinoma, and teratoma.

Phosphorylated by ATM at Thr-363 following ionizing radiation (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2 (By similarity).
Phosphorylation by PKC/PRKCZ at Ser-399 in isoform 2 promotes metformin (or peroxynitrite)-induced nuclear export of STK11 and activation of AMPK. UV radiation-induced phosphorylation at Thr-363 mediates CDKN1A degradation (By similarity).
Acetylated. Deacetylation at Lys-48 enhances cytoplasmic localization and kinase activity in vitro.