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Mouse Anti-SUPT5H Recombinant Antibody (17/DSIF) (CBMAB-D2258-YC)

Provided herein is a Mouse monoclonal antibody, which binds to SPT5 Homolog, DSIF Elongation Factor Subunit (SUPT5H). The antibody can be used for immunoassay techniques, such as WB, IF.
See all SUPT5H antibodies

Summary

Host Animal
Mouse
Specificity
Human, Dog, Mouse, Rat
Clone
17/DSIF
Antibody Isotype
IgG1
Application
WB, IF

Basic Information

Immunogen
Human DSIF a.a.866-985
Specificity
Human, Dog, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
suppressor of Ty 5 homolog (S. cerevisiae)
Introduction
SUPT5H has ubiquitous expression in testis (RPKM 64.5), bone marrow (RPKM 32.4) and 25 other tissues.
Entrez Gene ID
Human6829
Mouse20924
Rat308472
Dog476462
UniProt ID
HumanO00267
MouseO55201
RatE9PTB2
DogA0A8I3RS47
Alternative Names
SPT5; SPT5H; Tat-CT1
Function
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Biological Process
Biological Process negative regulation of DNA-templated transcription, elongationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of DNA-templated transcription, elongationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of macroautophagyManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of DNA-templated transcriptionManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Biological Process transcription elongation by RNA polymerase II promoterManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
PTM
Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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