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Rabbit Anti-TAF5 Recombinant Antibody (CBYJT-1879) (CBMAB-T0950-YJ)

Provided herein is a Rabbit monoclonal antibody, which binds to TAF5 (TATA-Box Binding Protein Associated Factor 5). The antibody can be used for immunoassay techniques, such as WB, IP, FC.
See all TAF5 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Rat, Mouse
Clone
CBYJT-1879
Antibody Isotype
IgG
Application
WB, IP, FC

Basic Information

Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TAF5 aa 700 to the C-terminus. The exact sequence is proprietary.
Specificity
Human, Rat, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 700-C-terminus

Target

Full Name
TATA-Box Binding Protein Associated Factor 5
Introduction
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF5 is an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. TAF5 interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex.
Entrez Gene ID
Human6877
Mouse226182
Rat294018
UniProt ID
HumanQ15542
MouseQ8C092
RatD3ZH66
Alternative Names
TATA-Box Binding Protein Associated Factor 5; TAF5 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 100kDa; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, D, 100kD; Transcription Initiation Factor TFIID 100 KDa Subunit; TAF(II)100; TAFII-100
Function
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473).
TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473).
The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473, PubMed:8758937, PubMed:8942982, PubMed:9045704).
The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473).
TAF5 is involved in two modules of TFIID, in TFIID-A together with TAF3 and TBP, and in TFIID-B with TAF8 (PubMed:33795473).
Involved in contacts between TFIID and TFIIF in the PIC (PubMed:33795473).
Biological Process
Biological Process DNA-templated transcription initiationIDA:UniProtKB1 Publication
Biological Process histone H3 acetylationIDA:ComplexPortal1 Publication
Biological Process monoubiquitinated histone deubiquitinationIDA:ComplexPortal1 Publication
Biological Process monoubiquitinated histone H2A deubiquitinationIDA:ComplexPortal1 Publication
Biological Process mRNA transcription by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process positive regulation of DNA-templated transcriptionIC:ComplexPortal1 Publication
Biological Process positive regulation of transcription initiation by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process protein phosphorylationIDA:ComplexPortal1 Publication
Biological Process regulation of DNA repairIC:ComplexPortal1 Publication
Biological Process regulation of transcription by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process RNA polymerase II preinitiation complex assemblyIPI:ComplexPortal1 Publication
Biological Process transcription initiation at RNA polymerase II promoterIDA:UniProtKB2 Publications
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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