Mouse Anti-TAF6 Recombinant Antibody (CBYJT-1887) (CBMAB-T0960-YJ)

TATA-Box Binding Protein Associated Factor 6

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID.

TATA-Box Binding Protein Associated Factor 6; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, E, 70/85kD; TAF6 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 80kDa; Transcription Initiation Factor TFIID 70 KDa Subunit; RNA Polymerase II TBP-Associated Factor Subunit E; TAF(II)70; TAF(II)80; TAFII-70; TAFII-80; TAFII70

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473).
TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473).
The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473).
The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473).
TAF6 homodimer connects TFIID modules, forming a rigid core (PubMed:33795473).
Isoform 4
Transcriptional regulator which acts primarily as a positive regulator of transcription (PubMed:20096117, PubMed:29358700).
Recruited to the promoters of a number of genes including GADD45A and CDKN1A/p21, leading to transcriptional up-regulation and subsequent induction of apoptosis (PubMed:11583621).
Also up-regulates expression of other genes including GCNA/ACRC, HES1 and IFFO1 (PubMed:18628956).
In contrast, down-regulates transcription of MDM2 (PubMed:11583621).
Acts as a transcriptional coactivator to enhance transcription of TP53/p53-responsive genes such as DUSP1 (PubMed:20096117).
Can also activate transcription and apoptosis independently of TP53 (PubMed:18628956).
Drives apoptosis via the intrinsic apoptotic pathway by up-regulating apoptosis effectors such as BCL2L11/BIM and PMAIP1/NOXA (PubMed:29358700).

Biological Process apoptotic processIEA:UniProtKB-KW
Biological Process DNA-templated transcription initiationIDA:UniProtKB1 Publication
Biological Process histone H3 acetylationIDA:ComplexPortal1 Publication
Biological Process monoubiquitinated histone deubiquitinationIDA:ComplexPortal1 Publication
Biological Process monoubiquitinated histone H2A deubiquitinationIDA:ComplexPortal1 Publication
Biological Process mRNA transcription by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process negative regulation of cell cycleIDA:UniProtKB1 Publication
Biological Process negative regulation of cell population proliferationIDA:UniProtKB
Biological Process positive regulation of apoptotic processIDA:UniProtKB
Biological Process positive regulation of DNA-templated transcriptionIC:ComplexPortal1 Publication
Biological Process positive regulation of intrinsic apoptotic signaling pathwayIDA:UniProtKB
Biological Process positive regulation of transcription initiation by RNA polymerase IIIDA:UniProtKB1 Publication
Biological Process protein phosphorylationIDA:ComplexPortal1 Publication
Biological Process regulation of DNA repairIC:ComplexPortal1 Publication
Biological Process regulation of transcription by RNA polymerase IIIDA:ComplexPortal1 Publication
Biological Process RNA polymerase II preinitiation complex assemblyIPI:ComplexPortal1 Publication
Biological Process transcription by RNA polymerase IINAS:UniProtKB1 Publication
Biological Process transcription initiation at RNA polymerase II promoterIDA:UniProtKB1 Publication

Nucleus
Isoform 4
Nucleus

Alazami-Yuan syndrome (ALYUS):
An autosomal recessive syndrome reminiscent of Cornelia de Lange syndrome and characterized by delayed psychomotor development with intellectual disability, hypotonia, microcephaly, short stature, poor speech, and dysmorphic features.

Isoform 4
In cells undergoing apoptosis, cleaved in a caspase-dependent manner to produce a 40 kDa product.