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Mouse Anti-TET1 Recombinant Antibody (4F4) (CBMAB-A9021-LY)

The product is antibody recognizes TET1. The antibody 4F4 immunoassay techniques such as: WB, ELISA.
See all TET1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4F4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
TET1 (NP_085128.1, 2038 a.a. ~ 2136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
tet oncogene 1
Entrez Gene ID
80312
UniProt ID
Q8NFU7
Alternative Names
CXXC6; FLJ10839; FLJ41442; KIAA1676; LCX; bA119F7.1
Function
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation (PubMed:19372391, PubMed:21496894).
Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC) (PubMed:21778364).
In addition to its role in DNA demethylation, plays a more general role in chromatin regulation by recruiting histone modifying protein complexes to alter histone marks and chromatin accessibility, leading to both activation and repression of gene expression (PubMed:33833093).
Plays therefore a role in many biological processes and diseases, including stem cell maintenance, T and B-cell development, inflammation regulation, genomic imprinting, neural activity or DNA repair (PubMed:31278917).
Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an important role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034).
Plays an essential role in the protection and maintenance of transcriptional and developmental programs together with QSER1 to inhibit the binding of DNMT3A/3B and therefore de novo methylation (PubMed:33833093).
Biological Process
Biological Process 5-methylcytosine catabolic processSource:InterPro
Biological Process chromatin organizationSource:UniProtKB-KW
Biological Process DNA demethylationSource:UniProtKB1 Publication
Biological Process inner cell mass cell differentiationSource:UniProtKB
Biological Process negative regulation of cell migrationSource:ComplexPortal1 Publication
Biological Process negative regulation of DNA methylation-dependent heterochromatin assemblySource:UniProtKB1 Publication
Biological Process negative regulation of stem cell population maintenanceSource:ComplexPortal1 Publication
Biological Process negative regulation of transcription by RNA polymerase IISource:ComplexPortal1 Publication
Biological Process negative regulation of transforming growth factor beta receptor signaling pathwaySource:ComplexPortal1 Publication
Biological Process oxidative demethylationSource:GO_Central1 Publication
Biological Process positive regulation of cell population proliferationSource:UniProtKB1 Publication
Biological Process positive regulation of histone methylationSource:UniProtKB1 Publication
Biological Process positive regulation of stem cell population maintenanceSource:ComplexPortal1 Publication
Biological Process positive regulation of transcription by RNA polymerase IISource:UniProtKB
Biological Process protein O-linked glycosylationSource:UniProtKB
Biological Process stem cell population maintenanceSource:UniProtKB
Cellular Location
Nucleus
Chromosome
Involvement in disease
A chromosomal aberration involving TET1 may be a cause of acute leukemias (PubMed:12646957). Translocation t(10;11)(q22;q23) with KMT2A/MLL1. This is a rare chromosomal translocation 5' KMT2A/MLL1-TET1 3' (PubMed:12124344, PubMed:12646957).
PTM
Glycosylated. Interaction with OGT leads to GlcNAcylation (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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