Mouse Anti-TFB2M Recombinant Antibody (CBYJT-2646) (CBMAB-T1825-YJ)

Name: Mouse Anti-TFB2M Recombinant Antibody (CBYJT-2646)
SKU: CBMAB-T1825-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-2646

Application

ELISA, WB

Immunogen

Partial recombinant corresponding to aa297-397 from human TFB2M (NP_071761) with GST tag. MW of the GST tag alone is 26kD

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 297-397

More Infomation

Target

Full Name

transcription factor B2, mitochondrial

Introduction

TFB2M (Transcription Factor B2, Mitochondrial) is a Protein Coding gene. Among its related pathways are Organelle biogenesis and maintenance and Gene Expression. Gene Ontology (GO) annotations related to TFB2M include rRNA methyltransferase activity.

Entrez Gene ID

64216

UniProt ID

Q9H5Q4

Alternative Names

Transcription Factor B2, Mitochondrial; S-Adenosylmethionine-6-N, N-Adenosyl(RRNA) Dimethyltransferase 2; Hepatitis C Virus NS5A-Transactivated Protein 5; Mitochondrial Transcription Factor B2; Mitochondrial 12S RRNA Dimethylase 2; HCV NS5A-Transactivated Protein 5; H-MtTFB2; H-MtTFB

Function

S-adenosyl-L-methionine-dependent rRNA methyltransferase which may methylate two specific adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA (Probable). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603, PubMed:12068295, PubMed:20410300, PubMed:15526033).
In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:29149603, PubMed:15526033).
Stimulates transcription independently of the methyltransferase activity (PubMed:12897151).

Biological Process

Biological Process mitochondrial transcriptionSource:GO_Central1 Publication
Biological Process regulation of DNA-templated transcriptionSource:InterPro
Biological Process rRNA methylationSource:GO_Central1 Publication
Biological Process transcription initiation at mitochondrial promoterSource:UniProtKB2 Publications

Cellular Location

Mitochondrion

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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