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Mouse Anti-TFE3 Recombinant Antibody (IHC672) (CBMAB-MA242-YC)

Provided herein is a Mouse monoclonal antibody against Transcription Factor Binding To IGHM Enhancer 3. The antibody can be used for immunoassay techniques, such as IHC-P, ELISA, WB, IHC. And it gave a positive signal in Testes, Melanoma and Xp11.2 Translocation Renal Cell Carcinoma.
See all TFE3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
IHC672
Antibody Isotype
IgG1
Application
IHC-P, ELISA, WB, IHC (1:100 - 1:200)

Basic Information

Immunogen
Recombinant Human TFE3
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
Tris buffer pH 7.3 - 7.7, 1% BSA, < 0.1% Sodium Azide
Storage
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Target

Full Name
Transcription Factor Binding To IGHM Enhancer 3
Introduction
TFE3 is a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes.
Entrez Gene ID
7030
UniProt ID
P19532
Alternative Names
Transcription Factor Binding To IGHM Enhancer 3; Class E Basic Helix-Loop-Helix Protein 33; Transcription Factor E Family, Member A; BHLHe33; Transcription Factor For Immunoglobulin Heavy-Chain Enhancer 3; Transcription Factor For IgH Enhancer;
Function
Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response (PubMed:2338243, PubMed:29146937, PubMed:30733432, PubMed:31672913).
Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity).
Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation (PubMed:31672913).
Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in nuclear localization and transcription factor activity (PubMed:31672913).
Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB; mTOR-dependent TFE3 cytosolic retention and inactivation promotes exit from pluripotency (By similarity).
Required to maintain the naive pluripotent state of hematopoietic stem cell; mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency (PubMed:30733432).
TFE3 activity is also involved in the inhibition of neuronal progenitor differentiation (By similarity).
Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue (By similarity).
In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity (By similarity).
Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer (PubMed:2338243).
It also binds very well to a USF/MLTF site (PubMed:2338243).
May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).
Biological Process
Biological Process adaptive immune responseSource:UniProtKB-KW
Biological Process humoral immune responseSource:UniProtKB
Biological Process negative regulation of cold-induced thermogenesisSource:YuBioLabBy Similarity
Biological Process positive regulation of brown fat cell differentiationSource:UniProtKB
Biological Process positive regulation of cell adhesionSource:UniProtKB1 Publication
Biological Process positive regulation of DNA-templated transcriptionSource:UniProtKB1 Publication
Biological Process positive regulation of transcription by RNA polymerase IISource:UniProtKB1 Publication
Biological Process regulation of osteoclast differentiationSource:InterPro
Biological Process regulation of transcription by RNA polymerase IISource:GO_Central1 Publication
Cellular Location
Cytoplasm, cytosol
Nucleus
When nutrients are present, phosphorylation by MTOR prevents nuclear translocation and activity (PubMed:22692423, PubMed:30733432).
Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:22692423, PubMed:30733432).
PTM
Sumoylated; does not affect dimerization with MITF.
Phosphorylation by MTOR regulates its subcellular location and activity (PubMed:21209915, PubMed:30733432).
When nutrients are present, phosphorylation by MTOR promotes retention in the cytosol (PubMed:30733432).
Inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:30733432).
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For research use only. Not intended for any clinical use.

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