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Mouse Anti-TONSL Recombinant Antibody (CBWJN-1384) (CBMAB-N2314-WJ)

This product is a Mouse antibody that recognizes TONSL. The antibody CBWJN-1384 can be used for immunoassay techniques such as: WB.
See all TONSL antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBWJN-1384
Antibody Isotype
IgG1
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
tonsoku-like, DNA repair protein
Introduction
TONSL (Tonsoku Like, DNA Repair Protein) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include transcription corepressor activity and histone binding. An important paralog of this gene is ANKRD31.
Entrez Gene ID
4796
UniProt ID
Q96HA7
Alternative Names
IKBR, NFKBIL2
Function
Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks (PubMed:21055983, PubMed:21055984, PubMed:21055985, PubMed:21113133, PubMed:26527279, PubMed:27797818, PubMed:29478807, PubMed:27338793, PubMed:30773278).
The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication (PubMed:21055983, PubMed:21055984, PubMed:21055985).
It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA): the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs (PubMed:21055983, PubMed:21055984, PubMed:21055985, PubMed:27797818, PubMed:29478807).
Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination (PubMed:27797818, PubMed:29478807).
Within the complex, TONSL acts as histone reader, which recognizes and binds newly synthesized histones following their replacement by histone chaperones (PubMed:29478807, PubMed:27338793).
Specifically binds histone H4 lacking methylation at 'Lys-20' (H4K20me0) and histone H3.1 (PubMed:27338793).
Biological Process
Biological Process double-strand break repair via homologous recombination Source:UniProtKB2 Publications
Biological Process replication fork processing Source:UniProtKB3 Publications
Cellular Location
Nucleus
Chromosome
Cytoplasm
Mainly nuclear (PubMed:21055983, PubMed:21055984).
Localizes to DNA damage sites, accumulates at stressed replication forks (PubMed:21055983, PubMed:21055984, PubMed:26527279, PubMed:27338793).
Recruited to stalled or collapsed replication forks following histone replacement by histone chaperones ASF1A and the CAF-1 complex: TONSL acts as histone reader that recognizes and binds newly synthesized histones (PubMed:29478807).
Involvement in disease
Spondyloepimetaphyseal dysplasia, sponastrime type (SEMDSP):
An autosomal recessive bone disease characterized by spine abnormalities, mid-face hypoplasia with a depressed nasal bridge, and striation of the metaphyses. Additional features include disproportionate short stature with exaggerated lumbar lordosis, scoliosis, coxa vara, limited elbow extension, small dysplastic epiphyses, childhood cataracts, short dental roots, and hypogammaglobulinemia. Disease severity and clinical manifestations are variable. Some patients have intellectual disability.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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