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Mouse Anti-TOPBP1 Recombinant Antibody (CBYJT-4219) (CBMAB-T3683-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TOPBP1 (DNA Topoisomerase II Binding Protein 1). The antibody can be used for immunoassay techniques, such as ICC, WB.
See all TOPBP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-4219
Antibody Isotype
IgG2b
Application
ICC, WB

Basic Information

Immunogen
Recombinant corresponding to aa1192-1621 from human TOP2B expressed in E. coli (Q02880)
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, Trehalose
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1192-1621

Target

Full Name
TOPBP1 Gene(Protein Coding) DNA Topoisomerase II Binding Protein 1
Introduction
TOPBP1 is a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Entrez Gene ID
11073
UniProt ID
Q92547
Alternative Names
DNA Topoisomerase II Binding Protein 1; DNA Topoisomerase II-Beta-Binding Protein 1; Topoisomerase (DNA) II Binding Protein 1; DNA Topoisomerase II-Binding Protein 1; DNA Topoisomerase 2-Binding Protein 1; KIAA0259; TOP2BP1; TopBP1
Function
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR (PubMed:16530042).
Biological Process
Biological Process cellular response to DNA damage stimulus Source:UniProtKB1 Publication
Biological Process DNA metabolic process Source:ProtInc1 Publication
Biological Process DNA repair Source:UniProtKB1 Publication
Biological Process DNA replication initiation Source:GO_Central1 Publication
Biological Process homologous recombination Source:ComplexPortal1 Publication
Biological Process mitotic DNA replication checkpoint signaling Source:GO_Central1 Publication
Biological Process mitotic G2 DNA damage checkpoint signaling Source:GO_Central1 Publication
Biological Process response to ionizing radiation Source:UniProtKB1 Publication
Cellular Location
Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, spindle pole
Chromosome
Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AX at unsynapsed chromosome cores during prophase (By similarity).
Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner.
PTM
Phosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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