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Rat Anti-Traf2 Recombinant Antibody (CBYJT-4504) (CBMAB-T4009-YJ)

Provided herein is a Rat monoclonal antibody, which binds to Traf2 (TNF Receptor-Associated Factor 2). The antibody can be used for immunoassay techniques, such as IP, WB.
See all Traf2 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYJT-4504
Antibody Isotype
IgG2a
Application
IP, WB

Basic Information

Immunogen
Recombinant mouse full-length TRAF2
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.2, 50% Glycerol
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TNF receptor-associated factor 2
Introduction
Traf2 is an adapter molecule that regulates the activation of NF-kappa-B and JNK. It functions in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of an E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity).
Entrez Gene ID
UniProt ID
Alternative Names
AI325259
Function
Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
Biological Process
Activation of NF-kappaB-inducing kinase activityManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to nitric oxideIEA:Ensembl
Interleukin-17-mediated signaling pathwayIEA:Ensembl
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stressManual Assertion Based On ExperimentTAS:ParkinsonsUK-UCL
mRNA stabilizationIEA:Ensembl
Negative regulation of glial cell apoptotic processIEA:Ensembl
Negative regulation of neuron deathManual Assertion Based On ExperimentTAS:ParkinsonsUK-UCL
Positive regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of extrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of I-kappaB phosphorylationIEA:Ensembl
Positive regulation of interleukin-2 productionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of JNK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of JUN kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of T cell cytokine productionManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of tumor necrosis factor-mediated signaling pathwayIEA:Ensembl
Programmed necrotic cell deathIEA:Ensembl
Protein autoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein catabolic processIEA:Ensembl
Protein K63-linked ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein-containing complex assemblyISS:BHF-UCL
Regulation of apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIBA:GO_Central
Regulation of immunoglobulin productionIEA:Ensembl
Regulation of protein-containing complex assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Response to endoplasmic reticulum stress1 PublicationNAS:ParkinsonsUK-UCL
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Signal transduction involved in regulation of gene expressionIEA:Ensembl
Tumor necrosis factor-mediated signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
PTM
Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.
Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Ubiquination is inhibited by LRRC19; inhibits proteasomal degradation (PubMed:25026888).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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