Mouse Anti-TRIM24 Recombinant Antibody (CBYJT-4714) (CBMAB-T4251-YJ)

Name: Mouse Anti-TRIM24 Recombinant Antibody (CBYJT-4714)
SKU: CBMAB-T4251-YJ
Rating: 5 (1 reviews)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBYJT-4714

Application

Immunogen

Human recombinant protein fragment corresponding to amino acids 706-1016 of human TRIM24 (NP_003843) produced in E. coli

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 706-1016

More Infomation

Target

Full Name

TRIM24

Introduction

TRIM24 mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. It localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. It is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region.

Entrez Gene ID

8805

UniProt ID

O15164

Alternative Names

Tripartite Motif Containing 24; RING-Type E3 Ubiquitin Transferase TIF1-Alpha; Transcriptional Intermediary Factor 1; E3 Ubiquitin-Protein Ligase TRIM24; RING Finger Protein 82; TIF1-Alpha; RNF82; TIF1A; TIF1; Transcription Intermediary Factor 1-Alpha

Function

Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation (PubMed:24820418).
Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity).
Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway (PubMed:32324863).
Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3 (PubMed:33724611).

Biological Process

Biological Process calcium ion homeostasis Source:Ensembl
Biological Process cellular response to estrogen stimulus Source:UniProtKB1 Publication
Biological Process epithelial cell proliferation Source:Ensembl
Biological Process negative regulation of DNA-templated transcription Source:Ensembl
Biological Process negative regulation of epithelial cell proliferation Source:Ensembl
Biological Process positive regulation of gene expression Source:Ensembl
Biological Process protein autophosphorylation Source:Ensembl
Biological Process protein catabolic process Source:UniProtKB1 Publication
Biological Process protein ubiquitination Source:UniProtKB1 Publication
Biological Process regulation of apoptotic process Source:UniProtKB1 Publication
Biological Process regulation of protein stability Source:UniProtKB1 Publication
Biological Process regulation of signal transduction by p53 class mediator Source:Ensembl
Biological Process regulation of vitamin D receptor signaling pathway Source:Ensembl
Biological Process transcription by RNA polymerase II Source:ProtInc1 Publication

Cellular Location

Nucleus
Cytoplasm
Mitochondrion
Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. Translocated from nucleus to mitochondria to mediate antiviral immunity (PubMed:32324863).

Involvement in disease

A chromosomal aberration involving TRIM24/TIF1 is found in papillary thyroid carcinomas (PTCs). Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.

PTM

Phosphorylated at Ser-768 by ATM kinase induces ubiquitination and degradation during DNA damage.
Sumoylated.
Undergoes ubiquitination-mediated degradation in response to DNA damage.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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