Mouse Anti-TSEN2 Recombinant Antibody (CBYJT-4995) (CBMAB-T4552-YJ)

Name: Mouse Anti-TSEN2 Recombinant Antibody (CBYJT-4995)
SKU: CBMAB-T4552-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYJT-4995

Application

WB, IHC-P, P-ELISA

Immunogen

Synthetic peptide (please contact us for details)

Specificity

Human, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

10 mM PBS, pH 7.4, 10 mg/mL BSA, 25% Glycerol

Preservative

0.03% Proclin 300

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

TRNA Splicing Endonuclease Subunit 2

Introduction

TSEN2 is one of the subunits of the tRNA splicing endonuclease. TSEN2 catalyzes the first step in RNA splicing which is the removal of introns. Mutations in TSEN2 have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4.

Entrez Gene ID

Human	80746
Rat	312649

UniProt ID

Human	Q8NCE0
Rat	Q5M954

Alternative Names

TRNA Splicing Endonuclease Subunit 2; TSEN2 TRNA Splicing Endonuclease Subunit; TRNA-Intron Endonuclease Sen2; HsSen2; SEN2; TRNA Splicing Endonuclease 2 Homolog (SEN2, S. Cerevisiae); TRNA Splicing Endonuclease 2 Homolog (S. Cerevisiae)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.

Biological Process

Biological Process mRNA processing Source:UniProtKB-KW
Biological Process tRNA splicing, via endonucleolytic cleavage and ligation Source:UniProtKB1 Publication
Biological Process tRNA-type intron splice site recognition and cleavage Source:GO_Central1 Publication

Cellular Location

Nucleus
Nucleus, nucleolus
May be transiently localized in the nucleolus.

Involvement in disease

Pontocerebellar hypoplasia 2B (PCH2B):
A disorder characterized by an abnormally small cerebellum and brainstem, and progressive microcephaly from birth combined with extrapyramidal dyskinesia. Severe chorea occurs and epilepsy is frequent. There are no signs of spinal cord anterior horn cells degeneration.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-TSEN2 Recombinant Antibody (CBYJT-4993) (CAT#: CBMAB-T4550-YJ)

Mouse Anti-TSEN2 Recombinant Antibody (CBYJT-4994) (CAT#: CBMAB-T4551-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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