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Mouse Anti-TSEN2 Recombinant Antibody (CBYJT-4995) (CBMAB-T4552-YJ)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBYJT-4995
Antibody Isotype
IgG
Application
WB, IHC-P, P-ELISA

Basic Information

Immunogen
Synthetic peptide (please contact us for details)
Specificity
Human, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
10 mM PBS, pH 7.4, 10 mg/mL BSA, 25% Glycerol
Preservative
0.03% Proclin 300
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
TRNA Splicing Endonuclease Subunit 2
Introduction
TSEN2 is one of the subunits of the tRNA splicing endonuclease. TSEN2 catalyzes the first step in RNA splicing which is the removal of introns. Mutations in TSEN2 have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4.
Entrez Gene ID
Human80746
Rat312649
UniProt ID
HumanQ8NCE0
RatQ5M954
Alternative Names
TRNA Splicing Endonuclease Subunit 2; TSEN2 TRNA Splicing Endonuclease Subunit; TRNA-Intron Endonuclease Sen2; HsSen2; SEN2; TRNA Splicing Endonuclease 2 Homolog (SEN2, S. Cerevisiae); TRNA Splicing Endonuclease 2 Homolog (S. Cerevisiae)
Function
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
Biological Process
Biological Process mRNA processing Source:UniProtKB-KW
Biological Process tRNA splicing, via endonucleolytic cleavage and ligation Source:UniProtKB1 Publication
Biological Process tRNA-type intron splice site recognition and cleavage Source:GO_Central1 Publication
Cellular Location
Nucleus
Nucleus, nucleolus
May be transiently localized in the nucleolus.
Involvement in disease
Pontocerebellar hypoplasia 2B (PCH2B):
A disorder characterized by an abnormally small cerebellum and brainstem, and progressive microcephaly from birth combined with extrapyramidal dyskinesia. Severe chorea occurs and epilepsy is frequent. There are no signs of spinal cord anterior horn cells degeneration.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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