Mouse Anti-TTC3 Recombinant Antibody (CBYJT-5125) (CBMAB-T4703-YJ)

Name: Mouse Anti-TTC3 Recombinant Antibody (CBYJT-5125)
SKU: CBMAB-T4703-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYJT-5125

Application

ELISA

Immunogen

Partial recombinant corresponding to aa1-110 from human TTC3 (NP_003307) with GST tag. MW of the GST tag alone is 26kD

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-110

More Infomation

Target

Full Name

tetratricopeptide repeat domain 3

Introduction

TTC3 (Tetratricopeptide Repeat Domain 3) is a Protein Coding gene. Diseases related to TTC3 include Retinitis Pigmentosa 58 and Down Syndrome. Gene Ontology (GO) annotations related to TTC3 include ligase activity and ubiquitin-protein transferase activity. An important paralog of TTC3 is DZIP3.

Entrez Gene ID

7267

UniProt ID

P53804

Alternative Names

Tetratricopeptide Repeat Domain 3; RING-Type E3 Ubiquitin Transferase TTC3; Tetratricopeptide Repeat Protein 3; RING Finger Protein 105; TPR Repeat Protein 3; TPR Repeat Protein D; RNF105

Function

E3 ubiquitin-protein ligase which catalyzes the formation of 'Lys-48'-polyubiquitin chains (PubMed:20059950, PubMed:30696809).
Mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus (PubMed:20059950).
Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation (PubMed:20059950).
Positively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation by mediating the ubiquitination and subsequent degradation of SMURF2 (PubMed:30696809).
Regulates neuronal differentiation by regulating actin remodeling and Golgi organization via a signaling cascade involving RHOA, CIT and ROCK (PubMed:17488780, PubMed:24695496).
Inhibits cell proliferation (PubMed:30203323).

Biological Process

Biological Process protein K48-linked ubiquitination Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication

Cellular Location

Nucleus
Cytoplasm
Golgi apparatus
Nuclear localization may be dependent on the proteolytic cleavage of full length protein in the cytoplasm (PubMed:30203323).
This cleavage may reveal an N-terminal nuclear localization signal, allowing N-terminal fragments to enter the nucleus (PubMed:30203323).

PTM

Phosphorylation on Ser-378 by Akt is required for ubiquitin ligase activity.
Proteolytically cleaved into differently sized N- and C-terminal fragments.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-TTC3 Recombinant Antibody (2D10) (CAT#: CBMAB-A9601-LY)

Isotype control

For research use only. Not intended for any clinical use.

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