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Mouse Anti-TYW1 Recombinant Antibody (CBYJT-5571) (CBMAB-T5204-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to TYW1 (TRNA-YW Synthesizing Protein 1 Homolog). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all TYW1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-5571
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
TYW1 (AAH51888, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MDPSADTWDL FSPLISLWIN RFYIYLGFAV SISLWICVQI VIKTQGKNLQ EKSVPKAAQD LMTNGYVSLQ EKDIFVSGVK IFYGSQTGTA KGFATVLAEA VTSLDLPVAI INLKEYDPDD HLIEEVTSKN VCVFLVATYT DGLPTESAEW FCKWLEEASI DFRFGKTYLK GMRYAVFGLG NSAYASHFNK VGKNVDKWLW MLGAHRVMSR GEGDCDVVKS KHGSIEADFR AWKTKFISQL QALQKGERKK SCGGHCKKGK CESHQHGSEE REEGSHEQDE LHHRDTEEEE PFESSSEEEF GGEDHQSLNS IVDVEDLGKI MDHVKKEKRE KEQQEEKSGL FRNMGRNEDG ERRAMITPAL REALTKQVHA PRERRLLQTH ILWN
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-384

Target

Full Name
TRNA-YW Synthesizing Protein 1 Homolog
Introduction
Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of TYW1 is essential for the synthesis of wybutosine.
Entrez Gene ID
55253
UniProt ID
Q9NV66
Alternative Names
TRNA-YW Synthesizing Protein 1 Homolog; Radical S-Adenosyl Methionine And Flavodoxin Domain-Containing Protein 1; Radical S-Adenosyl Methionine And Flavodoxin Domains 1; TRNA Wybutosine-Synthesizing Protein 1 Homolog; RSAFD1; S-Adenosyl-L-Methionine-Dependent TRNA 4-Demethylwyosine Synthase; TRNA-YW Synthesizing Protein 1 Homolog A (S. Cerevisiae)
Function
Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity).
Biological Process
Biological Process wybutosine biosynthetic process Source:GO_Central1 Publication
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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